锌指蛋白217在胰腺癌组织中的表达及临床意义

Expression and clinical significance of zinc finger protein 217 in human pancreatic cancer

目的：探讨锌指蛋白217（ zinc finger protein 217，ZNF217）在胰腺癌中的表达及临床意义。方法收集2011年4月至2014年5月间解放军117医院及瑞安市人民医院外科手术切除的43例胰腺癌及对应癌旁组织，实时定量PCR（ qRT-PCR）检测ZNF217 mRNA在胰腺癌及癌旁组织中的表达；免疫组织化学染色检测ZNF217蛋白表达，统计分析ZNF217蛋白与肿瘤临床病理资料间的相关性。结果 ZNF217 mR-NA在胰腺癌组织中表达水平为6.911±0.139，对应癌旁组织为1.372±0.205，差异有统计学意义（ t＝3.011，P＝0.013）；胰腺癌组织中ZNF217阳性表达率为72.09％（31／43），癌旁组织中ZNF217阳性表达率仅为16.28％（7／43），差异具有统计学意义（P＜0.001）。胰腺癌组织中ZNF217蛋白高表达与神经浸润、肿瘤体积增大、淋巴结转移及高TNM分期呈显著正相关（ P＜0.05）。结论 ZNF217 mRNA及蛋白在胰腺癌组织中表达上调并与肿瘤恶性临床病理特征有关。 ZNF217可能成为胰腺癌早期诊断的重要标志物及分子靶向治疗的有效靶点之一。

Objective To investigate the expression and clinical significance of zinc finger protein 217 （ZNF217）in human pancreatic cancer.Methods 43 cases with pancreatic cancer undergoing surgery in the PLA 117 Hospital and People＇s Hospital of Ruian City from Apr .2011 to May.2014 were enrolled in the study . The pancreatic cancer and the corresponding tumoradjacent tissues were collected .Real-time quantitative PCR （qRT-PCR）was applied to detect ZNF217 mRNA expression in pancreatic cancer （n=43）and the corresponding tumor-adjacent normal tissues.The protein expression of ZNF217 was measured by immunohistochemistry（IHC）. The relationship between the expression of ZNF 217 and clinical features was analyzed by pearson chisquare test . Results The expression level of ZNF217 mRNA and protein was significantly higher in pancreatic cancer tissues than in adjacent normal tissues（P〈0.05）.The high expression of ZNF217 protein was positively correlated with perineural invasion, tumor size, lymphatic metastasis and advanced TNM stage （P〈0.05）.Conclusions The expression of ZNF217 is significantly higher in pancreatic cancer tissues than in tumor-adjacent normal tissues , and the upregulation of ZNF 217 is associated with clinicopathological features of tumor malignance .ZNF217 may become a new marker and effective therapeutic target in early diagnosis of pancreatic cancer .