摘要
目的探讨血清基质金属蛋白酶-3(matrix metalloproteinases-3,MMP-3)与类风湿关节炎(rheumatoid arthritis,RA)及其炎症反应的相关性。方法用免疫比浊法(immunoturbidimetric assays)检测146例RA患者(RA组)及232例非RA人群(对照组)血清MMP-3水平;对两组MMP-3结果进行ROC曲线分析;分析RA患者血清MMP-3与反映RA患者炎症活动程度临床指标的关系。结果 RA组血清MMP-3浓度为170.00±19.36ng/mL,明显高于对照组的47.92±1.98ng/mL,差异有统计学意义(P<0.001)。血清MMP-3诊断RA的ROC曲线下面积为0.731,取诊断界限值为71.65ng/mL时,敏感度为58.9%,特异度为88.79%。RA组患者血清MMP-3水平与其他RA诊断以及反应RA炎症反应的指标,如C反应蛋白(C-reaction protein,CRP)、前白蛋白(prealbumin,PA)、血沉(erythrocyte sedimentation rate,ESR)、血小板(platelet)以及白细胞计数(WBC)均具有显著相关性,P值均<0.001。结论RA患者血清MMP-3水平显著高于正常人,其水平与反映RA患者炎症活动程度临床指标CRP、PA、ESR、PLT以及WBC具有显著相关性。类风湿性关节炎患者血清MMP-3水平与类风湿性关节炎的炎症活动性有关。
Objective To investigate the relationship between serum matrix metalloproteinase-3 and rheumatoid arthritis. Methods The serum MMP-3 level in 146 patients with RA and 232 non RA patients were detected by immunoturbidimetry. The receiver operating Curve （ROC） analysis was carried out to get the cut- off value. The relationship between MMP-3 and other clinical biochemical indices were analyzed by multiple regressions. Results The serum MMP-3 concentration in RA group（ 170 ±19.36 ng/mL） was significantly higher than that of control group（47.92 ± 1.98 ng/mL,P 〈0.001 ）. The optimal diagnostic cut-off value for MMP-3 was 71.65 ng/mL. The sensitivity and specificity were 58.9% and 88.79% respectively at this cut-off value. The serum concentration of MMP-3 in RA patients was significantly correlated with inflammation and activity indices such as C-reaction protein, prealbumin, erythrocyte sedimentation rate, platelet, white blood cell count（ P 〈 0. 001 ）. Conclusion The serum MMP-3 levels in patients with rheumatoid arthritis is correlated to inflammatory activity.
出处
《标记免疫分析与临床》
CAS
2014年第6期636-639,共4页
Labeled Immunoassays and Clinical Medicine
