不同剂量致敏原对小鼠过敏性哮喘模型特异性抗体和炎性细胞因子的影响

Influence of different dose of sensitinogen on serum specific antibody and inflammatory cytokines in allergic asthma model in mice

目的 观察不同剂量致敏原对小鼠过敏性哮喘模型血清特异性抗体IgE、IgG2a和白细胞介素-4（IL-4）、白细胞介素-5（IL-5）、白细胞介素-12（IL-12）、γ-干扰素（IFN-γ）等炎性细胞因子的影响.方法 45只雌性BALB小鼠随机分为4组,空白对照组腹腔注射不含鸡卵蛋白（OVA）的PBS缓冲液,低、中、高剂量组分别注射含10、100、1 000 μg OVA的PBS缓冲液,第15 ～20天以雾化吸入OVA生理盐水激发致敏,制备过敏性哮喘模型.结果 各给药组均出现不同程度炎细胞浸润,低剂量组最为显著;低、中、高剂量组血清IgE、IL-4、IL-5水平呈递减趋势,组间差异显著（P＜0.01）,且均显著高于对照组（P＜0.01）;各实验组血清IgG2a、IL-12及IFN-γ水平呈递增趋势,组间差异显著（P＜0.01）,且与对照组差异显著（P＜0.05或P＜0.01）.结论 不同剂量的OVA可能影响小鼠过敏性哮喘模型所产生的细胞因子类型,低剂量OVA造模的效果最为显著,高剂量OVA可能导致小鼠发生免疫耐受.

Objective To observe the influence of different dose of snetitinogen on IgE, IgG2a, IL-4, IL-5, IL-12 and IFN-γ in allergic asthma model in mice. Methods A total of 45 female BALB mice were divided into 4 groups, of which blank control group was given intraperitoneal injection of PBS buffer solution not containing OVA while low, middle and high dose groups were injected PNS buffer solution containing 10, 100 and 1 000 p.g OVA, respectively. Allergic asthma models were prepared by aerosol inhalation of OVA normal saline （NS） for sensitization. Results Different degree of inflammatory cell infiltration appeared in medication administration groups, and most obvious in low-dose group. IgE and IL-4 and IL-5 levels in low, middle and high dose groups showed a trend of decline and were significantly higher than those of control group （P 〈 0.01）. IgG2a, IL-12 and IFN-γ levels showed a increasing trend and there were significant differences among groups （ P 〈 0.05, or P 〈 0.0O1 ）. Conclusion Different dose of OVA may affect cytokine type caused by allergic asthma model in mice, and high-dose OVA might lead to immune tolerance in mice.