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鞘内注射TRESK基因重组腺病毒对坐骨神经分支选择性损伤大鼠的镇痛作用 被引量：1

Effects of recombinant adenovirus vector containing TRESK gene by intrathecal injection on hyperalgesia of sciatic nerve branch selective injury rats
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摘要 目的研究鞘内注射TRESK基因重组腺病毒（p Ad/CMV/V5-DEST-TRESK）对坐骨神经分支选择性损伤（SNI）大鼠痛觉过敏的影响。方法雄性SD大鼠36只,随机分为6组（n=6）：Ⅰ组为空白对照组;Ⅱ组为假手术组;Ⅲ组为SNI模型组;Ⅳ组大鼠制备SNI模型后鞘内单次注射生理盐水25μl;Ⅴ组和Ⅵ组大鼠制备SNI模型后,鞘内分别单次注射滴度为1.31×109TU/ml的阴性腺病毒和p Ad/CMV/V5-DEST-TRESK。各组大鼠于术前1 d及术后1 d、3 d、5 d、7 d、14d分别测定机械痛阈和热痛阈;术后14 d检测L4,5术侧背根神经节（DRG）的TRESK蛋白表达。结果Ⅲ组、Ⅳ组、Ⅴ组、Ⅵ组术后1 d、3 d、5 d、7 d、14 d的机械痛阈明显低于Ⅰ组（P〈0.05）,Ⅲ组、Ⅳ组、Ⅴ组5 d、7 d、14 d的机械痛阈明显低于Ⅵ组（P〈0.05）;与术前比较,Ⅲ组、Ⅳ组、Ⅴ组、Ⅵ组术后1-14 d的机械痛阈明显降低（P〈0.05）。Ⅲ组、Ⅳ组、Ⅴ组、Ⅵ组L4,5术侧DRG的TRESK蛋白表达明显低于Ⅰ组（P〈0.05）,Ⅵ组L4,5术侧DRG的TRESK蛋白表达明显高于Ⅲ组、Ⅳ组、Ⅴ组（P〈0.05）。结论鞘内注射p Ad/CMV/V5-DEST-TRESK可上调SNI大鼠DRG的TRESK蛋白表达,减轻SNI大鼠的痛觉过敏。 Objective To investigate the effects of recombinant adenovirus vector containing TRESK gene（ pAD/CMV/V5 -DEST-TRESK）by intrathecal injection on hyperalgesia of sciatic nerve branch selective injury（ SNI）rats. Methods Thirty six SD male rats were randomly divided into six groups,six rats in each groups：control group（ Group I）;sham operation group（ GroupⅡ）;SNI group（ GroupⅢ）. SNI rats were given respectively single intrathecal injected 25μl physiological saline. The negative adenovirus and pAD/CMV/V5-DEST-TRESK in Group Ⅳ,Group Ⅴ and GroupⅥ,the titer of adenovirus was 1. 31 ＆#215; 109 TU/ml. Rats in each groups were determined by mechanical threshold （MWT）and heat pain threshold respectively（TWL）at 1d before operation and 1,3,5,7,14 d after operation. The protein of TRESK in the side of L4,5 DRG were determined at 14 d after operation. Results The MWT were significantly lower in GroupⅢ,Ⅳ,ⅤandⅥthan in Group I at 1d before operation and 1,3,5,7,14 d after operation（ P ﹤0. 05）. The MWT were significantly lower in GroupⅢ,ⅣandⅤthan in GroupⅥat 5,7,14 d after operation（ P ﹤0. 05）. The MWT in GroupⅢ,Ⅳ,ⅤandⅥwere significantly lower at 1,3,5,7,14 day after operation than at 1 day before operation（ P ﹤0. 05）. The protein of TRESK in DRG in GroupⅢ,Ⅳ,ⅤandⅥwere significantly lower than in Group I （ P ﹤0. 05）. The protein of TRESK in DRG in GroupⅥwere significantly higher than in GroupⅢ,ⅣandⅤ（ P ﹤0. 05）. Conclusion Intrathecal injection with the recombinant adenovirus vector containing TRESK gene（ pAD/CMV/V5-DEST-TRESK ）can up-regulate the protein of TRESK in DRG and alleviate the hyperalgesia of SNI rats.
作者 高润兴 王汉兵 仲吉英 周俊
机构地区 佛山市第一人民医院麻醉科
出处 《临床和实验医学杂志》 2014年第23期1929-1932,共4页 Journal of Clinical and Experimental Medicine
关键词 大鼠 神经病理性疼痛 TRESK基因重组腺病毒 鞘内注射 背根神经节 钾通道 Rats Neuropathie pain Recombinant adenovirus vector containing TRESK gene Intrathecal injection Dorsal root ganglion Potassium channel
分类号 R614 [医药卫生—麻醉学]
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参考文献13

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共引文献7

同被引文献16

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临床和实验医学杂志
2014年 第23期

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