白血病中PTEN mRNA的表达状态研究

Detection of PTEN mRNA expression in leukemia

目的利用实时PCR技术检测急性粒细胞白血病(AML)、急性淋巴细胞白血病(ALL)和慢性淋巴细胞白血病(CLL)中的PTEN mRNA的表达状况。方法收集病例AML21例,ALL3例和CLL23例,按照相应的情况进行分组,比较各组PTEN mRNA在不同情况下的表达状态。结果 AML和ALL组的目的基因Ct值没有明显差异(P>0.05),且这两组的Ct值要明显高于CLL组的Ct值(P<0.05),这三个组的Ct值要明显高于对照组的Ct值(P<0.05)。AML组和ALL组的初治组Ct值要高于缓解组的Ct值,CLL组的初治组的Ct值和缓解组的Ct值没有明显差异(P>0.05)。AML、ALL和CLL组的高肿瘤负荷组的Ct值要明显高于低肿瘤负荷组的Ct值(P<0.05)。结论通过实验确定了三种病例的PTEN基因表达状况,为疾病的预判和转归可能提供了辅助的可靠地途径。

Objective The aim of the present study was to detect the expression of PTEN mRNA in AML,ALL and CLL using real time PCR. Methods Case AML（21）,ALL（3）and CLL（23）are grouped according to the respective situation. The PTEN mRNA expression status in different situations were compared to find out the difference. Results The gene of AML and ALL Ct values did not differ significantly（ P﹥0. 05）. These Ct value of two groups was significantly higher than the Ct value of CLL group（ P ﹤0. 05）. The three groups Ct values were significantly higher than the Ct values（ P ﹤0. 05）. The Ct values of untreated AML and ALL groups were higher than the Ct values remission group. Meanwhile no significant difference was found in untreated and remittent group of CLL（ P ﹥0. 05）. The Ct value of the high tumor burden group in AML,ALL and CLL was significantly higher than that of the Ct value of low tumor burden group（ P ﹤0. 05）. Conclusion Our study indicated that detection of PTEN gene expression may provide a reliable way to predict the outcome of the disease.