具有双重抗菌作用的两性离子水凝胶

Zwitterionic Hydrogel with Dual Antibacterial Action

合成了一种甲基丙烯酰胺类两性离子单体,采用自由基聚合法制备具有双重抗菌作用的水凝胶。利用1 H-NMR和FT-IR表征单体和水凝胶,考察了水凝胶释放水杨酸的水解曲线。以导入绿色荧光蛋白的大肠杆菌为试验菌,荧光显微镜下观察水凝胶表面的细菌黏附情况;以金黄色葡萄球菌和大肠杆菌为试验菌,研究水凝胶的抗菌性能。结果表明,该水凝胶可有效防止细菌黏附并且通过释放水杨酸实现抗菌性能。

A novel methylacrylamide derivative zwitterionic monomer was synthesized, and hydrogel with dual antibacterial action was prepared via radical polymerization. The obtained monomer and hydrogel were characterized by 1 H-NMR and FT-IR. The hydrolysis curve of the hydrogel released salicylic acid was also investigated. E. coli which contained the green fluorescent protein was applied to evaluate the bacterial adhesion efficacy of the hydrogel surface. The antibacterial activity of the hydrogel was investigated against both E. coli and S. aureus. Results showed that the hydrogel not only prevented bacteria surface adhesion but also released salicylic acid to achieve antibacterial action.