多重MSP检测早期鼻咽癌甲基化基因的初步探索

A tentative exploration of multiplex methylation specific PCR for early diagnosis of nasopharyngeal carcinoma

目的:探讨多重MSP(multiplex-MSP)检测基因甲基化在鼻咽癌早期诊断中的作用。方法:通过MSP方法对35例Ⅰ期鼻咽癌标本18个基因的甲基化状态进行验证;同时通过条件摸索建立多重MSP方法,尝试实现多个基因甲基化状态的快速检测。结果:甲基化率超过50%的基因有12个;多重MSP检测中仅1组合的非甲基化特异性产物(U片段)扩增效果没有达到预期结果 ,余3个组合电泳结果与甲基化特异性PCR相符。结论:采取合适的组合进行多重MSP扩增可获得理想结果 ,其结果与MSP以及甲基化芯片扫描结果相似,是一种效率高,成本较低的实验方法。

Objective To explore the effect of multiplex methylation specific PCR for early diagno~ is of nasopharyngeal carcinoma. Methods The methylation status of 18 genes were verified by MSP from 35 early nasopharyngeal carcinoma andmuhiplex-MSP was established through the optimized working condition for rapid detection of several genes＇ methylation status. Results The methylation rate of 12 genes exceeds 50%. The methylation specific products in only one group （Segment U） did reach the expected results while those from the other three groups were consistent with the MSP＇s. Conclusion Muhiplex-MSP is an efficient and low-cost melhod, and it can produce ideal experimental results similar to those by the MSP and DNA microarrays.