人参白术药对配伍前后化学成分变化研究

Content changes of chemical constituents in Ginseng Radix and Atractylodis Macrocephalae Rhizoma before and after compatibility

目的以人参Ginseng Radix和白术Atractylodis Macrocephalae Rhizoma药对为研究对象,分析人参、白术单煎液与合煎液的化学成分变化,以期从化学成分角度阐明人参-白术药对配伍的科学内涵。方法采用HPLC法,Hypersil ODS（250mm×4.6 mm,5μm）色谱柱;流动相为乙腈-水溶液;梯度洗脱程序：0~35 min,5%~35%乙腈;35~40 min,35%乙腈保持;40~80 min,35%~95%乙腈;检测波长203 nm;柱温25℃;体积流量1 m L/min。结果经HPLC指纹图谱分析人参、白术单煎液与合煎液化学成分存在明显差异,人参-白术合煎液中的人参皂苷Rg1、Re、Rf、Rd和白术内酯I的量明显增加,并出现了1个新色谱峰,其他成分无明显变化。结论人参与白术配伍可促进人参中皂苷类成分的溶出,并产生了1个新的色谱峰,可能是该药对配伍的科学依据之一。

Objective To discuss the synergistic mechanism of compatibility of Ginseng Radix（GR） and Atractylodis Macrocephalae Rhizoma（AMR）, and to determine the compounds in GR and AMR before and after compatibility using HPLC method. Methods Hypersil ODS column（250 mm × 4.6 mm, 5 μm） was adopted. The mobile phase was acetonitrile（A） and water（B） for gradient elution（0—35 min, 5%—35% A; 35—40 min, 35% A; 40—80 min, 35%—95% A）. The detection wavelength was set at 203 nm. The column temperature was 25 ℃. The flow rate was 1 m L/min. Results The HPLC analysis suggested the extracts before and after compatibility were different on contents of GR and AMR. In GR and AMR co-decoction, the contents of ginsenosides Rg1, Re, Rf, Rd, and butenolide I increased obviously, and a new chromatographic peak appeared. There was no obvious change in other components. Conclusion The synergistic mechanism of the compatibility of GR and AMR can be proved with the increased dissolution of ginsenoside and a new chromatographic peak.