ISC-HPLC法同时测定桃红四物汤中酚类物质

Simultaneous determination of phenols in Taohong Siwu Decoction by ISC-HPLC

目的建立离子抑制高效液相色谱（ISC-HPLC）法测定桃红四物汤中酚类物质的方法。方法采用ISC-HPLC法测定桃红四物汤中羟基红花黄色素A、阿魏酸、没食子酸、原儿茶酸和绿原酸的量,分光光度法测定桃红四物汤中总酚酸的量。结果桃红四物汤中羟基红花黄色素A、阿魏酸、没食子酸、原儿茶酸和绿原酸分别在5.00~80.00、1.25~20.00、0.60~9.60、0.90~14.40、1.00~16.00μg/m L与峰面积呈良好线性关系;平均回收率分别为98.50%、97.17%、97.17%、98.45%、97.16%（n=9）。结论 ISC-HPLC方法简便、准确、快速、重复性好,可用于桃红四物汤中酚类物质的质量控制。

Objective To determine the contents of phenols in Taohong Siwu Decoction（TSD） by ISC-HPLC. Methods The ISC-HPLC was used to determine the contents of hydroxysafflor yellow A, ferulic acid, gallic acid, protocatechuic acid, and chlorogenic acid. Spectrophotometric method was used to determine the contents of total phenolic acids. Results The contents of hydroxysafflor yellow A, ferulic acid, gallic acid, protocatechuic acid, and chlorogenic acid in TSD had good linear relationships with the peak area in the ranges of 5.00—80.00, 1.25—20.00, 0.60—9.60, 0.90—14.40, and 1.00—16.00 μg/m L, respectively, and the average recoveries（n = 9） were 95.82%, 94.98%, 96.52%, 97.16%, and 98.14%, respectively. Conclusion ISC-HPLC method is simple, rapid, accurate, and reproducible, and can be used for the quality control of TSD.