斑马鱼耳聋基因Gfi与POU4f3的关联性

The relation between Gfi and Pou4f3 by zebrafish

目的运用转基因斑马鱼探讨信号蛋白Gfi与POU4f3之间关系。方法阻止POU4f3在鱼受精卵中表达,构建该基因缺陷的转基因鱼,分析缺失该基因的斑马鱼形态及Gfi的表达。结果 POU4f3的缺陷程度与鱼神经组织发育的程度有明显相关性,与Gfi表达也有明显相关性。结论 POU4f3对Gfi表达有调控作用,二者与神经组织发育有明显相关性。

Objective Using zebrafish for study the relation between Gfi and Pou4f3 by zebrafish. Methods Deter the Pou4f3 expression and build up the transgenic zebrafish. Results There is an obvious correlation between Gfi and Pou4f3, which can affect the never tissue development. Conclusion Growth factor independence 1 （Gfi1） as a likely target gene regulated by Pou4f3.