不同长春花中3种生物碱的含量测定

Determination of three kinds of important alkaloids in Catharanthus roseus from ten different habitats

目的：建立快速准确的长春花内生物碱含量测定方法,为寻找高含量品种提供方法和依据。方法：采用反相高效液相色谱法（RP-HPLC）,对收集到的10个长春花品种中的文多灵、长春质碱、长春碱含量进行了测定,色谱柱：Dikma Diamonsil-C18（4.6mm×250mm,5μm）;流动相为A∶0.7%二乙胺（磷酸调整p H至7.2）;B：乙腈：甲醇=25：40。A∶B=35∶65;流速1ml/min。柱温35℃。检测波长：280nm。结果：文多灵：Y=2.00×10^6X＋5.46×10^4,r=0.9999,线性范围：0.2-4μg;长春质碱：Y=2.28×10^6X＋8.31×10^4,r=0.9999,线性范围：0.2-4μg;长春碱：Y=1.76×10^6X-7.17×10^4,r=0.9999,线性范围：0.2-4μg。文多灵含量最高的是1号品种,长春质碱含量最高的是3号品种,长春碱含量最高的是2号品种,三种生物碱总和最高的是3号品种。结论：该方法简便、快速,分析准确,适合对长春花内主要生物碱做高通量的筛选。综合考虑总碱的含量和最重要的长春碱的含量,1号品种适合作为代谢调控的本体。

Objective To establish a fast and accurate method of determination of alkaloids in Catharanthus roseus for finding high-content varieties.Method To determinate the contents of Vinblastine,Catharanthine and Vindoline in the 10 varieties of Catharan-thus roseus by RP -HPLC,HPLC method is performed on Dikma Diamonsil -C18 （4. 6mm ×250mm,5μm） column,mobile phase：A：0. 7% diethylamine （pH 7. 2 adjusted by phosphoric acid ）;B：acetonitrile∶methanol =25∶40.A∶B =35∶65;flow rate is 1ml/min.Column temperature is 35 ℃.Detected at 280nm.Results Vindoline：Y=2. 00 ×10^6X＋5. 46 ×10^4,r=0. 9999,linear range：0. 2-4μg;Catharanthine：Y=2. 28×10^6X＋8. 31×10^4,r=0. 9999,linear：0. 2 -4μg;Vinblastine：Y=1. 76×10^6X-7. 17×10^4,r=0. 9999,linear range：0. 2-4μg.The species of the highest content of vindoline is the 1st,Ca-tharanthine is the 3rd,vinblastine is the 2nd species,the highest sum of three alkaloids is on the 3rd varieties.Conclusion This method is simple,reliable,repeatable,and is suitable for High-throughput screening of the Alkaloids content in Catharanthus roseus. The 1 st varieties is suitable as the body of metabolic regulation.