大鼠双侧颈总动脉阻断法致全脑缺血模型的改良研究

Modified two vessel blocking method of acute global ischemia model in rats

目的改良大鼠双侧颈总动脉永久性阻断模型(2VO)以适应急性全脑缺血研究。方法平行制作双侧颈总、颈内、颈外动脉永久性阻断(6VO)和2VO全脑缺血模型及假手术组,比较2种模型的死亡率、行为学评分、海马CA1区细胞坏死率、凋亡率及炎症因子的差异。结果 6VO组24 h内死亡率(40%)与2VO组(0)、假手术组相比均明显增高,各个时间点行为学评分增高,神经元坏死、凋亡增加且炎症因子表达增高(均P<0.05)。结论改良模型可加重大鼠脑损伤,适合急性全脑缺血研究。

Objective To adapt to the acute cerebral ischemia research,we modified the permanent bilateral common carotid artery occlusion（ 2VO） rat model.Methods Global ischemia models were established by permanent ligation of bilateral common,internal,external carotid arteries（ 6VO） and the 2VO method respectively.The sham group were done simultaneously.Then the differences among three groups in mortality,behavioral score tests,pathological morphology,the expression of inflammatory factor by immunohistochemical method,and the percentage of cell apoptosis were compared.Results Twenty- four hours after operations,the mortality of 6VO group（ 40%） was obviously higher than 2VO group（ 0）.The results of behavioral score,necrosis rate of neurons,the expression of inflammatory factors and cell apoptosis in hippocampus CA1 area in 6VO group were significantly higher than 2VO group and sham group（ P〈0.05）.Conclusion The modified 6VO model is an ideal way to observe acute global ischemic injury in rats.