基于荧光素汞荧光法结合光纤传感-微顺序注射-阀上实验室测定肠灌流液中H2S

Fluorescein Mercury Combined with Optical Sensing Micro Sequential Injection Lab-on-Valve for Determination of H_2S in Intestinal Perfusate

建立基于光纤传感技术结合微顺序注射-阀上实验室荧光猝灭测定肠灌流液中H2S含量方法。本研究进行单因素考察,确定以100μL 0.1mol/L NaOH溶液为载液,荧光素汞浓度为5.0×10^-5mol/L、体积50μL、进样体积50μL、流通池检测流速25μL/s,根据H2S猝灭荧光素汞521 nm处荧光,对样品中H2S浓度进行测定。本方法检测H2S的浓度范围为5.0×10^-6～8.0×10^-5mol/L,检出限为5.4×10^-7mol/L。测定肠灌流液中H2S含量为3.8×10^-5mol/L,RSD=3.1%（n=3）。本方法能有效在线测定样品中H2S含量,为实时在线测定生物样品中的H2S含量奠定基础。

A fuorescence quenching method was established for the determination of H2S in intestinal perfusate by optical fiber sensing technology combined with micro sequential injection lab-on-valve （ μSIAlov）. In the experiment, 100 μL of 0. 1 mol/L NaOH was used as the carrier, and 50 μL of 5.0×10^-5 mol/L fluorescein mercury and 50 μL of sample were selected for the determination. The detection flow rate at the flowcell was 25 μL/s. According to H2S quenching fluorescence at 521 nm, the concentration of H2S in the sample was determined. The detected concentration range of H2S was 5.0 × 10^-6-8. 0 × 10^-5 mol/L, and the detection limit was 5.4×10^-7 mol/L. Detection result of H2S in intestinal perfusion was 3.8×10^-5 mol/L with 3.1% RSD （n=3）. This method can be used for the determination of H2S effectively in the samples, which lay the foundation for real-time online measurement of H2 S in biological samples.