IL-17F及IL-17A基因多态性与中国四川汉族人群多发性硬化的相关性

Association of interleukin-17Fand interleukin-17Agene polymorphisms with multiple sclerosis in a Sichuan Han population

目的探讨中国四川汉族人群中白细胞介素(interleukin,IL)-17A多态位点rs2275913及IL-17F基因多态位点rs763780与多发性硬化(multiple sclerosis,MS)发病的相关性。方法收集川北医学院附属医院神经内科就诊的MS患者126例,以176名性别年龄相匹配的健康体检者为对照,利用聚合酶链式反应-限制性片段长度多态性方法来检测rs2275913及rs763780多态位点的等位基因及基因型频率分布,并进行统计学分析。结果 MS患者IL-17F基因rs763780位点TT基因型频率(84.92%)高于健康对照组(71.01%),差异有统计学意义(校正后P=0.036)。患者T等位基因频率(92.06%)亦高于正常人(84.66%),差异有统计学意义(校正后P=0.048)。MS患者IL-17A位点rs2275913的基因型频率及等位基因频率与健康对照者相比无统计学差异(P>0.05)。结论 IL-17F基因多态位点rs763780与MS相关,TT基因型及T等位基因可能是中国四川汉族人群MS发生的危险因素。IL-17A位点rs2275913同四川汉族人MS发病无相关。

Objective To discuss the genetic association of interleukin（IL）-17 polymorphisms with multiple sclerosis（MS）in Sichuan Han population.Methods Two single nucleotide polymorphisms（SNPs）,rs763780 of IL-17 Fgene and rs2275913 of IL-17 A gene were genotyped in 126 MS patients and 176 healthy controls by polymerase chain reaction-restriction fragment length polymorphism method（PCR-RFLP）.All control subjects and patients were ethnically and geographically matched.Allele and genotype frequencies distributions between patients and controls were compared using the Chi-Square test.Results A significantly increased rs763780 TT genotype frequency（corrected P=0.036）and T allele frequency（corrected P=0.048）were found in MS patients in comparison with controls.The genotypic and allelic frequencies of rs2275913 in IL-17 Agene were not different between patients with MS and controls.Conclusions These results suggest that rs763780 is associated with multiple sclerosis in a Sichuan Han population,TT genotype and T allele of rs763780 may confer higher risk of MS,whereas rs2275913 in IL-17 Agene is not associated with MS.