MIP-2、MIP-1α和IL-6在小鼠血流感染中的变化规律实验研究

Study on the Variation of MIP-2, MIP-1α and IL-6 in mice with bloodstream infection

目的探讨巨噬细胞炎性蛋白2(macrophage inflammatory protein-2,MIP-2)、巨噬细胞炎性蛋白1α(macrophage inflammatory protein-1 Alpha,MIP-1α)和白细胞介素6(interleukin-6,IL-6)在金黄色葡萄球菌和大肠埃希菌感染时的变化规律及在鉴别诊断金黄色葡萄球菌和大肠埃希菌感染的价值。方法将216只ICR小鼠随机分为3组,各组72只。尾静脉注射大肠埃希菌和金黄色葡萄球菌建立小鼠血流感染模型,生理盐水注射建立阴性对照。共9个时间点,各时间点每组8只小鼠。分别在感染0.5 h、1 h、3 h、6 h、12 h、1 d、2 d、3 d、5 d后采血,检测血液白细胞计数(WBC),血清中MIP-2、MIP-1α和IL-6浓度。结果 1大肠埃希菌组和金黄色葡萄球菌组小鼠在感染0.5 h后MIP-2、MIP-1α和IL-6均明显升高,各指标峰值分别为(13 246.8±865.37)pg/ml、(699.79±36.36)pg/ml、(25 080.32±1 263.58)pg/ml、(3 483.22±169.84)pg/ml和(1 171.98±104.49)pg/ml、(94.71±14.38)pg/ml,大肠埃希菌感染组显著高于金黄色葡萄球菌感染组;2MIP-2、MIP-1α和IL-6在大肠埃希菌感染时的ROC曲线下面积(0.962、0.967、0.962)与金黄色葡萄球菌感染时的(0.877、0.889、0.885)的差异有统计学意义(P<0.05),并且两组之间差异也有统计学意义(P<0.05)。结论 MIP-2、MIP-1α和IL-6在炎症早期明显升高,在鉴别大肠埃希菌和金黄色葡萄球菌引发的感染均具有统计学意义。

Objective To explore the variation of Macrophage Inflammatory Protein-2, Macrophage Inflammatory Protein-1Alpha ,interleukin-6 in mice with blood infection and analyze the correlation between them. The value of MIP-2, MIP-1α and IL-6 in differential diagnosis of Staphylococcus aureus infection and Escherichia coli infection were evaluat-ed. Methods 216 mices were divided into three groups randomly. Models of mices with bloodstream infection were estab-lished by being challenged with Escherichia coli and Staphylococcus aureus by intravenous tail injection. Seventy-two mice were challenged with isotonic sodium chloride solution as controls. WBC, MIP-2, MIP-1α and IL-6 were determined at 0.5 h,1 h, 3 h, 6 h, 12 h, 1 d, 2 d, 3 d and 5d after infections （n=8 per group）. Results （1） MIP-2, MIP-1αand IL-6 all increased significantly at 0.5 h after infections and reached the peak at 6 h, （13 246.8±865.37）pg/ml, （595.4±28.3）pg/ml, （25 080.32±1 263.58）pg/ml, （2 509.13±272.05）pg/ml, （1 171.98±104.49）pg/ml, （94.71±14.38）pg/ml respectively. （2） The ar-eas under the ROC curve （AUC） of MIP-2, MIP-1α and IL-6 in two infecting groups were significantly different （P〈0.05）. Conclusion MIP-2, MIP-1α and IL-6 increase significantly early. All of them can be used to differentiate Es-cherichia coli and Staphylococcus aureus infections.