摘要
利用阴离子交换色谱和反相C_(18)色谱柱纯化菌株SIPI-2013406的代谢产物,获得纯度高于98%的化合物样品。采用质谱和核磁共振鉴定结构,推测该产物为富马酰-L-丙氨酸。产生菌经过形态和分子特征鉴定,确定菌株SIPI-2013406为产黄青霉种(Penicillium chrysogenum)。
One of the secondary metabolites of strain SIPI-2013406 was purified through anion exchange resin and reverse-phase C18 chromatographic column with purity over 98 %. Its structure was elucidated as fumaryl-L-alanine by MS and NMR analysis. After the morphological and molecular characterization, the strain SIPI-2013406 was identified as Penicillium chrysogenum.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2015年第1期20-23,共4页
Chinese Journal of Pharmaceuticals
基金
创新微生物药物高效筛选与发现技术平台(2012ZX9301002003)
关键词
富马酰-L-丙氨酸
产黄青霉
纯化
结构解析
fumaryl-L-alanine
Penicillium chrysogenum
purification
structure identification
