复合微生物菌剂发酵酒精糟生产蛋白饲料的研究

Study on protein feedstuff production with distiller grain fermented by complex microbial inoculants

通过设计不同菌种组合发酵酒精糟实验,选取最佳微生物菌种组合,进一步设计单因素实验和正交实验,确定最优菌剂配比及最佳发酵工艺。结果表明:酒精糟饲料的最佳发酵工艺为白地霉、热带假丝酵母、纤维单胞菌X6菌种配比1︰1︰2,菌剂接种量0.5%,发酵时间为3d。在此工艺条件下进行酒精糟固体发酵实验,其产物中真蛋白质质量分数可达30.22%,粗纤维质量分数降至7.27%。与原料糟相比,真蛋白质增长率与粗纤维降解率分别达到40.30%和44.12%;蛋氨酸、赖氨酸、苏氨酸总量提高了28.41%。

Different fermentation experiments were conducted to produce Distiller Grains（DGs）by using various combinations of strains.And the best microbial strain combination was selected as a result.The optimal ratio and fermentation process was further determined in the method of single factor and orthogonal experiments.In accordance with the results,the best fermentation technological condition for DG production was described as follows：1︰1︰2（Geotrichum candidum,Candida tropicalis,Cellulomonas X6）for bacteria strain ratio,0.5% of inoculation dose and fermentation for 3days.In addition,a solid fermentation experiment of GD was carried out with the same processing parameters.As a result,the pure protein content in the product reached to 30.22% whereas the crude fiber content was reduced to 7.27%.Comparing with raw materials for distiller grains,true protein increasing rate and crude fiber degradation rate were found at 40.30% and 44.12%respectively.The total amount of methionine,lysine and threonine was increased by 28.41%in the end.