犬细小病毒JL-M13株的分离鉴定及全基因组序列分析

Isolation,Identification and Sequence Analysis of Complete Genome of Canine Parvovirus JL-M13 Strain

将检测为犬细小病毒（CPV）阳性的病料经处理后接种F81细胞盲传3代并获得一株细小病毒,命名为JL-M13。应用重叠PCR技术从JL-M13中扩增出5条核苷酸序列,拼接后进行序列分析。结果表明,所获病毒核酸基因组大小为4 621bp,与其他犬细小病毒的基因相似性为98.2%-99.4%。系统发育树分析表明,JL-M13与CPV-2毒株（EF011664.1）相似率最高,与Type-2型犬细小病毒疫苗株相距较远。

We inoculated canine parvovirus positive samples into F 81 cells and isolated a virus strain named JL-M13 by three passages.Five nucleotide sequences were amplified from the DNA extracted from canine parvovirus wild strain by overlapping PCR.Splicing and analysis of the sequences showed that the genome length of the strain were 4621bp,the identity with other canine parvovirus was 98.2%-99.4%.Phyloge-netic tree analysis showed that the similarity rate of JL-M13 with CPV-2 （EF011664.1）was the highest and was significantly lower with canine parvovirus type-2 vaccine strain.