心房钠尿肽基因敲除小鼠的鉴定

Identification of Atrial Natriuretic Peptide Gene Knockout(ANP^(-/-))Mice

研究心房钠尿肽基因敲除小鼠(ANP-/-小鼠)的生物学及病理学特性。ANP-/-小鼠与C57BL/6小鼠饲养于SPF级环境中,观察小鼠的繁育状况及其生物学特性,选取合适时间处死小鼠,通过HE染色观察脏器形态及组织结构。ANP-/-小鼠的繁殖周期比C57BL/6长,产仔率较低;ANP-/-小鼠肝脏、肺脏、脾脏较C57BL/6小鼠的有明显炎症反应,但是C57BL/6小鼠未见明显的病理改变。提示ANP基因的缺失可能与炎症发生有关。说明ANP-/-小鼠繁殖较慢、产仔率低,且正常繁殖的普通小鼠不会引起器官的病理学改变。但作为一种在心血管、代谢疾病上的动物模型,并与炎症甚至肿瘤有可能的未知联系的转基因动物,其很有研究和探索价值。

To investigate the biological features of new transgenic mice （ANP^- /- mice） and compare its pathological features with normal mice.ANP^- /- mice and C57BL/6 mice （normal control mice） were housed in the same SPF environment to observe the multiplication and study their biological characteris-tics.The histological morphology and structure of organs were compared with normal mice.The ANP^- /-mice have longer reproductive cycle and lower birth rate ;Moreover,ANP^-/- mice show slightly higher in-flammatory response than C57BL/6 mice,suggesting that ANP gene may associated with inflammation. The reproduction and birth rate of ANP^- /- mice are slow,but as the animal model used in cardiovascular and metabolic disease,even inflammation or cancer,they have potential research and exploration value.