3个甘蔗与斑茅远缘杂交后代BC_1的染色体遗传分析

Genetic Analysis of Chromosome in 3 BC_1 Clones from the Distant Crossing Between Saccharum spp.and Erianthus arundinaceus

对3个甘蔗与斑茅远缘杂交后代BC1进行真实性鉴定及染色体核型分析,以探讨甘蔗与斑茅BC1的染色体传递方式。利用2对鉴定斑茅真实杂交后代的特异引物对3个甘蔗与斑茅BC1进行鉴定,采用根尖分生区细胞去壁低渗涂片法制片,显微拍照计数染色体数目,并进行染色体核型分析。3个BC1材料均为斑茅的真实杂交后代,崖城01-69体细胞染色体核型公式为2n=121=120 m+1 sm,其染色体按2n+n方式传递;崖城01-116的体细胞染色体核型公式为2n=122=118 m+4 sm,其染色体传递方式为2n+n;崖城01-134的体细胞染色体核型公式为2n=121=120 m+1 sm,其染色体传递为2n+n。推断甘蔗与斑茅BC1的染色体以2n+n的方式传递。

To explore the chromosome transmission, the karyotypes of 3 BC1 clones from the distant crossing between Saccharum spp. and Erianthus arundinaceus were analyzed. 3 BC1 clones were identified using 2 pairs of specific primers of true E. arundinaceus progenies. Chromosomes were prepared according to cell wall degradation hypotonic smear method, the chromosomes number of 3 BC1 clones was calculated and the karyotypes of 3 BCI clones were analyzed. 3 BC1 clones were true progenies of E. arundinaceus. The somatic chromosome karyotypic type of YC01-69 was 2n=121=120 m＋1 sm, following 2n＋n transmission; The somatic chromosome karyotypic type of YC01-116 was 2n=122=118 m＋4 sin, following 2n＋n transmission; The somatic chromosome karyotypic type of YC01-134 was 2n=121=120 m＋l sin, following 2n＋n transmission. The 2n＋n transmission of BC1 clones from Saccharum spp. and E. arundinaceus can be resumed.