基于慢性粒细胞白血病(CML)中血管内皮生长因子(VEGF)的高表达探讨大黄蛰虫丸对CML K562细胞VEGF表达的影响

Expression of Vascular Endothelial Growth Factor(VEGF) in Chronic Myelogenous Leukemia(CML) Patients and Influence of Serum Containing Dahuang Zhechong Pills on VEGF Expression in K562 Cells

【目的】通过观察大黄蛰虫丸对慢性粒细胞白血病(CML)K562细胞血管内皮生长因子(VEGF)表达的影响,探索应用抗血管新生药物治疗CML的新途径。【方法】采用酶联免疫吸附(ELISA)法,检测CML慢性期(38例)、急变期(11例)患者及正常人(9例)血清中VEGF的含量;并用SD大鼠制备大黄蛰虫丸含药血清,用ELISA法检测K562细胞的VEGF含量。【结果】与正常对照组比较,CML慢性期、急变期患者血清VEGF含量显著升高(P<0.05或P<0.01);与正常对照组比较,大黄蛰虫丸含药血清预处理K562细胞的VEGF蛋白分泌量显著下降(P<0.01),而且VEGF表达随着作用药物浓度的增大而递减。【结论】VEGF在CML慢性期、急变期的含量增高,可能与CML的发病、进展及预后有关;大黄蛰虫丸可抑制慢性粒细胞白血病K562细胞VEGF的表达,使蛋白表达量下降,且呈浓度依赖性。

Objective To observe the effect of serum containing Dahuang Zhechong Pills on vascular endothelial growth factor （ VEGF） expression in K562 cells of chronic myelogenous leukemia （ CML） and to develop new antiangiogenic drugs for CML treatment. Methods Enzyme-linked immunosorbent assay （ ELISA） was used for the detection of serum VEGF content in 38 CML patients, 11 acute myelogenous leukemia patients and 9 healthy volunteers. SD rats were used for the preparation of serum containing Dahuang Zhechong Pills, and then ELISA was applied for the detection of VEGF content in K562 cells pretreated with serum containing Dahuang Zhechong Pills. Results Compared with the healthy volunteers, myelogenous leukemia patients at chronic phase and acute phase had high serum VEGF content （ P〈0.05 or P〈0.01） . Compared with blank serum control group, the secretion of VEGF in K562 cells pretreated with serum containing Dahuang Zhechong Pills was decreased obviously （ P〈0.01） , and VEGF expression decreased with the increase of drug concentration. Conclusion VEGF content is increased in myelogenous leukemia patients at chronic phase and acute phase, which may be related to the pathogenesis, progress and prognosis of chronic myelogenous leukemia. Serum containing Dahuang Zhechong Pills can inhibit the expression of VEGF in chronic myelogenous leukemia K562 cells and decrease VEGF protein expression in concentration-dependent manner.