表里经配穴法对脑缺血再灌注损伤大鼠海马细胞凋亡及JNK信号通路的影响

Influence of Exterior-interior Meridian Acupoint Selection on Hippocampal Cell Apoptosis and Expression of c-jun N-terminal Kinase in Rats with Ischemia-reperfusion Injury

【目的】观察表里经配穴法对脑缺血再灌注损伤大鼠海马细胞凋亡及c-jun氨基末端激酶(JNK)信号通路的影响,探讨其对脑缺血再灌注损伤保护作用的可能机制。【方法】将120只SD大鼠随机分为假手术组、模型组、表里经配穴电针组、抑制剂组;参照Longa法复制局灶脑缺血再灌注模型,表里经配穴电针组取表里经配穴的足三里、三阴交和尺泽、合谷行电针治疗,每日1次。采用神经行为学评定各组大鼠不同时段神经行为,TUNEL法检测海马细胞凋亡指数,免疫组织化学法检测p-JNK表达。【结果】表里经配穴电针组、抑制剂组神经功能缺损评分均显著低于模型组(P<0.05);模型组凋亡指数显著高于假手术组(P<0.01),表里经配穴电针组和抑制剂组凋亡指数均显著低于模型组(P<0.05);模型组各时段p-JNK表达较假手术组显著增加(P<0.01),表里经配穴电针组、抑制剂组p-JNK表达较模型组显著降低(P<0.05)。【结论】表里经配穴法可不同程度地减轻缺血再灌注后大鼠的神经功能缺损,减少细胞凋亡,其机制可能与抑制JNK信号通路有关。

Objective To observe the influence of exterior-interior meridian acupoint selection on hippocampal cell apoptosis and c-Jun N-terminal kinase （ JNK） in ischemia-reperfusion injury rats, and to explore the possible protective mechanism for ischemia-reperfusion injury. Methods One hundred and twenty SD rats were randomly divided into sham-operation group （SH）, model group （I/R）, electroacupuncture （EA） group and JNK inhibitor SP600125 （ SP） group. The ischemia-reperfusion model was established by occlusion of middle cerebral artery with intraluminal thread. EA group was given EA on acupoints of Zusanli （ ST 36） , Sanyinjiao （ SP 6） , Chize （ LU 5） , Hegu （ LI 4） , once a day, based on the method of exterior-interior meridian acupoint selection. We observed the neurological behavioral changes by Longa neurological function standard, detected the activation of phosphorylated JNK （ p-JNK） by immunohistochemical method and examined the hippocampal apoptotic index by TUNEL method. Results The neurologic impairment scores of EA group and SP group were lower than I/R group （P〈0.05） . Apoptotic index of I/R group was higher than SH group （P〈0.01） and that of EA group and SP group was lower than I/R group （P 〈0.05） . The level of p-JNK in I/R group was higher than SH group （ P 〈0.01） and that of EA group and SP group was lower than I/R group （ P 〈0.05）. Conclusion Exterior-interior meridian acupoint selection therapy has certain effect on alleviating neurologic impairment and reducing apoptosis in ischemia- reperfusion injury rats, and the mechanism might be associated with the inhibition of the activation of JNK signaling pathway.