保心康联合常规药物干预对心力衰竭模型大鼠心功能及腺苷酸代谢的影响

Effects of Combination of Baoxinkang and Conventional Medicine Intervention on Cardiac Function and Adenylate Metabolism of Heart Failure Rats

【目的】观察保心康联合常规药物对慢性心力衰竭(CHF)模型大鼠心功能、腺苷酸代谢的影响,探讨保心康治疗CHF的增效作用。【方法】选用60只SD大鼠随机分为假手术组、模型组、保心康组、常规治疗组、常规治疗+保心康组、常规治疗+曲美他嗪组。采用腹主动脉缩窄术复制慢性心力衰竭大鼠模型。各治疗组分别给予保心康(1 020 mg/kg)、常规治疗(美托洛尔10 mg/kg、卡托普利5 mg/kg、地高辛0.022 5 mg/kg)、曲美他嗪(10 mg/kg)6周,以心脏彩超测定各组大鼠的心功能,并采用高效液相色谱法检测心肌组织腺苷酸(ATP、ADP、AMP)含量。总腺苷酸量(TAN)=ATP+ADP+AMP,能荷(EC)=(ATP+0.5×ADP)/TAN。【结果】心脏彩超显示常规治疗+保心康组大鼠的心脏小于模型组及常规治疗组,左室射血分数、心输出量显著高于模型组及常规治疗组,ATP、TAN、EC水平显著增高,ADP、AMP水平显著降低(均P<0.05或P<0.01)。常规治疗+保心康组与常规治疗+曲美他嗪组比较,心脏大小、左室射血分数、心输出量,以及ATP、ADP、AMP、TAN、EC水平差异均无统计学意义(P>0.05)。【结论】保心康可以改善CHF大鼠心肌的能量代谢障碍,保心康对CHF的常规药物治疗具有一定的增效作用。

Objective To evaluate the synergistic effect of Baoxinkang on chronic heart failure （ CHF） rats by observing the effect of the combination of Baoxinkang and conventional medicine intervention on cardiac function and adenylate metabolism. Methods Sixty Sprague-Dawley rats were randomly divided into 6 groups, namely＆nbsp;sham-operation group, model group, Baoxinkang group （ Baoxinkang 1 020 mg/kg） , conventional medicine intervention group （metoprolol 10 mg/kg, captopril 5 mg/kg, and digoxin 0.022 5 mg/kg）, combination group 1 （ conventional medicine intervention ＋ Baoxinkang 1 020 mg/kg） , and combination group 2 （ conventional medicine intervention ＋ trimetazidine 10 mg/kg） . Abdominal aora was constricted to establish CHF rat model. The rats except for the sham-operation group and model group were given the corresponding medicine according to the experimental design for 6 weeks. Echocardiography （ ECHO） was performed to evaluate the cardiac function of rats. High performance liquid chromatography was utilized to investigate the contents of myocardiac adenylate of adenosine triphosphate （ ATP） , adenosine diphosphate （ ADP） , adenosine monophosphate （AMP） . Total adenylate nucleotide pool （TAN） was equal to ATP＋ADP＋AMP, energy charge （EC） was equal to （ ATP＋0.5 ×ADP） /TAN. Results The results of ECHO showed that the heart size was reduced, left ejection fraction and cardiac output were increased in the combination group 1 compared with the model group and conventional medicine intervention group. The levels of ATP, TAN, and EC were significantly increased, whereas the levels of ADP and AMP were decreased in the combination group 1 （P0.05） . Conclusion Baoxinkang may have some synergistic effect on the improvement of CHF rat cardiac energy metabolism disorder treated by conventional medicine.