黄芩苷对脂多糖诱导的肠道上皮细胞凋亡及迁移能力的影响

Effect of Baicalin on LPS-induced Apoptosis and Cell Migration of Intestinal Epithelial Cells

【目的】探讨黄芩苷对脂多糖(LPS)诱导的IEC-6细胞凋亡及迁移能力的影响。【方法】以LPS诱导IEC-6细胞建立细胞损伤模型,采用四甲基偶唑盐(MTT)法和酶联免疫吸附(ELISA)法试剂盒分别检测LPS对IEC-6增殖及分泌炎症因子肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的影响;应用Hoechst 33258染色和流式细胞术观察和分析黄芩苷低、中、高剂量组(剂量分别为2.5、5.0、10.0μg/m L)对LPS诱导的IEC-6细胞核凋亡形态学改变及凋亡率的影响;应用细胞划痕实验检测黄芩苷对LPS诱导的IEC-6细胞迁移能力的影响。【结果】与对照组比较,1.0μg/m L LPS组IEC-6细胞生存率显著降低,TNF-α、IL-6分泌量及细胞凋亡率显著增加,细胞迁移能力显著下降(均P<0.05);与模型组比较,黄芩苷低、中、高剂量组凋亡细胞均不同程度减少,高剂量组细胞凋亡率显著降低(P<0.05),细胞迁移显著提高(均P<0.01)。【结论】黄芩苷可抑制LPS诱导的肠道上皮细胞的凋亡,并能提高其迁移能力。

Objective To investigate the effects of baicalin on the apoptosis and cell migration of intestinal epithelial cells 6 （IEC-6） induced by lipopolysaccharide （LPS） . Methods LPS was adopted to establish LPS-induced cells injury model. Methyl thiazolyl tetrazolium （ MTT） assay and Elisa kits were used to evaluate the effect of LPS on the tumor necrosis factor alpha （ TNF-α） and interleukin 6 （ IL-6） concentrations of IEC-6. Hoechst 33258 staining and flow cytometry were used to evaluate the effect of baicalin （ in the dosage of 2.5, 5.0, 10.0 μg/mL ） on the cell apoptosis. Cell migration was observed and calculated on a wounding model of IEC-6 cells induced by a pipette tip. Results Compared with the control group, the survival rate of IEC-6 in 1.0 μg·mL-1 LPS group was much lowered, TNF-α and IL-6 concentrations were significantly increased, the apoptotic rate of IEC-6 was increased and the cell migration activity was significantly decreased （ P〈0.05） . Compared with the model group, IEC-6 apoptosis was relieved to various degrees in baicalin groups, the apoptotic rate of IEC-6 was obviously decreased （P〈0.05） and cell migration activity was increased significantly （P〈0.01） in 10.0 μg·mL-1 baicalin group. Conclusion Baicalin has the capacity of protecting IEC-6 from LPS-induced injury and increasing cell migration activity.