榴莲皮提取物抗炎作用研究

Experimental Study of Anti-inflammation of Durian Peel Extract

【目的】探讨榴莲皮提取物(DPE)的抗炎效果及其作用机制。【方法】采用角叉菜胶致小鼠足跖肿胀及2,4-二硝基氟苯(2,4-DNFB)引起小鼠变应性接触性皮炎实验模型评价DPE的体内抗炎效果;采用四甲基偶氮唑盐(MTT)法以及脂多糖(LPS)诱导的RAW 264.7细胞炎症模型评估体外抗炎效果。【结果】在体内实验,与空白组比较,DPE各剂量组对角叉菜胶诱发的小鼠足跖肿胀抑制效果显著(P<0.01或P<0.001),DPE对2,4-DNFB所致小鼠变应性接触性皮炎也呈现明显的抑制作用,且作用效果具有较好的量效关系。在体外实验,在给定的浓度下DPE不影响细胞增殖;25 mg/L与50 mg/L DPE可有效地抑制炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、一氧化氮(NO)以及转录因子核因子-кВ(NF-кВ)含量,提高白细胞介素-10(IL-10)等抗炎因子含量。【结论】DPE具有较好的抗炎作用,其作用可能与DPE抑制NF-кВ信号通路相关。

Objective To investigate the anti-inflammatory effects and mechanism of durian peel extracts （DPE）. Methods The in vivo anti-inflammation effects of DPE were examined by carrageenin-induced mice paw edema test and allergic contact dermatitis test induced by 2, 4-DNFB. And the in vitro anti-inflammation effects of DPE were evaluated with methyl thiazolyl tetrazolium （ MTT） assay in RAW 264.7 cell model of inflammation induced by lipopolysaccharide （LPS）. Results The results of animal experiments showed that DPE groups could markedly relieve mice paw edema induced by carrageenin （ P〈0.01 or P〈0.001 compared with blank group）. DPE could effectively inhibit the allergic contact dermatitis induced by 2, 4-DNFB in mice, showing good dose-effect relationship. The results of in-vitro test showed that DPE at the given concentrations had no influence on RAW 264.7 cell proliferation. Tumor necrosis factor alpha （ TNF-α） , interleukin 6 （ IL-6） , interleukin 1 beta （IL-1β）, nitric oxide （NO） and nuclear factor kappaB （NF-кВ ） were observably inhibited, and anti-inflammatory cytokine IL-10 was enhanced by 25 and 50 mg/L of DPE. Conclusion DPE exert potential anti-inflammation effect, and the mechanism might be related to its inhibition of NF-кВsignal pathway.