秦皮素对苯肾上腺素诱导的大鼠心肌细胞肥大的影响

Effect of fraxetin on cardiomyocyte hypertrophy induced by phenylephrine

目的观察秦皮素对苯肾上腺素诱导的原代SD乳鼠心肌细胞肥大的影响。方法建立苯肾上腺素诱导的SD乳鼠原代心肌细胞肥大模型,观察秦皮素对心肌细胞肥大的影响;图像分析法计算心肌细胞面积;[3 H]-亮氨酸掺入法测试心肌细胞蛋白质合成速率;实时定量荧光PCR法检测心肌细胞Nrf2和肥大分子标志物心房钠尿肽、心房利尿肽的mRNA表达水平。结果(1)80μmol/L苯肾上腺素刺激心肌细胞48h能成功复制SD乳鼠心肌细胞肥大模型并伴随Nrf2表达量的增加;(2)秦皮素呈剂量依赖性地抑制苯肾上腺素刺激的SD乳鼠心肌细胞面积的增大、蛋白质合成速率的增加和肥大分子标志物的上调。结论秦皮素能显著抑制PE诱导的原代大鼠心肌细胞肥大。

Objective To investigate the effect of fraxetin on primary cardiomyocyte hypertrophy induced by phenylephrine . Methods Primary SD cardiomyocyte hypertrophy was induced by phenylephrine ,and we observed the effects of fraxetin on cardio‐myocyte hypertrophy induced by phenylephrine .Image‐ProPlus 5 measured the area of cardiomyocyte;[3 H ]‐leucine incorporation assay detected the protein synthesis rate of cardiomyocyte;Real‐time PCR measured the Nrf2 and molecular markers （ANP ,BNP） mRNA expression levels of cardiomyyocyte hypertrophy .Results （1）Primary neonate SD Cardiomyocyte hypertrophy model was successfully established by 80 μmol/L phenylephrine for 48 h ,and Nrf2 expression levels significantly increased in cardiomyocyte hypertrophy model;（2）The increase of cardiomyocyte area ,protein synthesis rate and molecular markers expression of cardiomyyo‐cyte hypertrophy were significantly inhibited by fraxetin in a dose‐dependent manner .Conclusion Fraxetin could significantly inhib‐it the cardiomyyocyte hypertrophy induced by PE .