大豆MYB转录因子基因GmMYB174的克隆及分子特性分析

Isolation and Molecular Characteristics Analysis of Soybean Transcription Factor Gene GmMYB174

克隆获得了大豆转录因子MYB基因GmMYB174,该基因序列全长1086 bp,编码361个氨基酸,属于MYB-related家族。生物信息学分析表明大豆GmMYB174与番茄、苜蓿、葡萄等物种同源性较高,序列分析表明包含2个保守的Trp(W)位点和1个保守基序SHAQKFF。亚细胞定位结果显示GmMYB174定位于细胞核中;组织表达量显示GmMYB174在多个组织均有表达,其中在上胚轴中表达量最高。启动子元件分析表明GmMYB174包含ABRE、MYB、MYC、LTRE、GT-1等逆境胁迫应答元件。实时荧光定量PCR分析表明Gm MYB174在干旱、盐、ABA处理下均有响应。因此,GmMYB174可能参与多种胁迫应答途径。

The full sequence of GmMYB174 was isolated from soybean, including 1086 bp and encoding 361 amino acids and belonged to MYB-related family. Bioinformatics analysis indicated that GmMYB174 shared high similarity with tomato, alfalfa and grape, containing 2 conserved locus and a motif of SHAQKFF. Subcellular localiza- tion analysis showed that GmMYB174 located in nucleus. Expression analysis showed that GmMYB174 expressed in many tissues and peaked in epicotyl. Promoter analysis indicated that GmMYBI74 contained ABRE, MYB, MYC, LTRE and GT-1. Q-RT-PCR showed that GmMYB174 involved in various abiotic stresses such as drought, salt, and ABA. Therefore, GmMYB174 might participated in multi-environmental stress responses.