高油酸花生(Arachis hypogaea L.)杂交后代ahFAD2B基因的分子标记辅助选择

Molecular Marker Assisted Selection of ahFAD2B Gene in High Oleate Peanut(Arachis hypogaea L.) Hybrids

本研究利用等位基因特异性PCR技术(AS-PCR,allele-specific PCR)对高油酸父本CTWE与4个低油酸母本组配的330个杂交后代进行分子鉴评,其中230个获得了539 bp的特异性条带。白沙1016×CTWE、海花1号×CTWE、冀0212-2×CTWE以及远杂9847×CTWE的真杂种百分率分别为83.3%、50.0%、57.1%和50.0%。本研究采用单粒近红外光谱分析法对F2:3家系进行检测,结果表明远杂9847×CTWE、白沙1016×CTWE、冀0212-2×CTWE以及海花1号×CTWE的F2:3家系中,全部为高油酸类型的家系分别为9个、8个、2个和3个,推断F2群体中,基因型为FAD2B-m/FAD2B-m的个体的比例为9.47%、4.17%、3.39%和3.37%。4个杂交组合高油酸性状的遗传在P=0.05水平上符合2对基因的遗传模式。本研究结果对于高油酸性状的分子鉴定、高油酸花生新品种的培育以及育种效率的提高具有一定的参考价值。

Allele-specific PCR（AS-PCR） was adopted to evaluate 330 hybrids from 4 hybridization combina- tions, which including one high-oleate male parent CTWE and four low-oleate female parents. The results showed that only 230 hybrids had specific 539 bp band. True hybrid percentage of Baisha 1016 × CTWE, Haihual × CTWE, Ji 0212-2 × CTWE,and Yuanza 9847 × CTWE were 83.3% ,50.0% ,57.1% ,and 50.0% respectively. High oleic F2：3 family lines of Yuanza 9847 × CTWE, Baisha 1016 × CTWE ,Ji 0212-2 × CTWE ,and Haihual × CTWE were 9, 8,2, and 3, respectively by near-infrared spectroscopy analysis. The deduced ratio of FAD2B-m/FAD2B-m in F2 population were 9.47% ,4.17% ,3.39% ,and 3.37% ,respectively. The genetic pattern of the high oleic trait of 4 hybridization combinations was all accord with two pairs of genes at P = 0.05 level. This study had certain reference value for molecular identification and new variety cultivation of high oleie peanut, and also could increase the effi- ciency of high oleic peanut breeding.