神经节苷脂和高压氧联用对脑缺血再灌注小鼠学习记忆能力的影响

Effects of Ganglioside and Hyperbaric Oxygenation on Learning and Memory Induced by Cerebral Ischemia reperfusion in Mice

目的：探讨神经节苷脂（ GM1）和高压氧（ HBO ）联合应用对脑缺血再灌注损伤小鼠学习记忆能力作用的影响。方法：将小鼠随机分为5组，假手术组、模型组、GM1组、HBO组和GM1＋HBO组。采用小鼠双侧颈总动脉夹闭缺血再灌注模型，＆amp;nbsp；GM1组和HBO＋GM1组分别腹腔注射GM1（10mg／kg／d），HBO组、假手术组和模型组分别给予相应体积生理盐水，每天1次，连用7天后进行抑制性回避反应及Y－水迷宫试验，检测小鼠学习记忆能力；采用HE染色观察海马CA1区锥体形态学，细胞数及密度变化。结果：回避反应能力试验：模型组小鼠24小时后3分钟内步入暗室次数明显增加，与假手术组比较具有显著性差异（p＜0．01）；GM1组、HBO组和HBO＋GM1组与模型组比较步入暗室的次数减少（p＜0．05，p＜0．01）；HBO＋GM1组小鼠步入暗室的次数可恢复至正常水平，分别与GM1组和HBO组比较具有统计学意义（ p＜0．05）。水迷宫试验：模型组小鼠24小时后10秒内到达坪台次数明显减少，与假手术组比较具有显著性差异（ p＜0．05）；HBO＋GM1组小鼠与模型组比较10秒内到达坪台次数基本恢复正常（p＜0．05）；HBO＋GM1组分别与GM1组和HBO组10秒内到达坪台次数也明显增加（p＜0．05）。海马区细胞数及密度增加，分别与GM1组、HBO组比其作用明显增加。结论：GM1与HBO联合应用对CIR所致的海马区组织形态学的改变及小鼠学习记忆功能障碍均具有保护作用，而且比GM1和HBO单独应用的作用更明显。

Objective：To investigate the effects of combined treatment hyperbaric oxygenation （ HBO ） with ganglioside （ GM1 ） on learning and memory impairment induced by brain ischemia reperfusion （ CIR） in mice.Methods：KunMing mice were randomly divided into 5 groups：sham group,model group, GM1 group, HBO group and GM1＋HBO group.The ischemia/reperfusion model following tran-sient cerebral ischemia was produced by clamping both common carotid arteries in mice .After brain ischemia for 15 min and reperfusion was given, GM1 group and HBO＋GM1 group were given with GM1 （10mg/kg/d, ip）, model group and HBO group were given 0.9%NS （10 ml/kg/d, ip）.After injected for 7 consecutive days , open field behavior, inhibitory avoidance response （IAR） and Y-water maze （ YWM） test methods were used to measure the changes of learning and memory ability , and variations of hippocampal CA 1 pyrami-dal cell morphology , cell number , and density was observed by hematoxylin -eosin （ HE） stain.Results： Avoidance response capacity test：the darkroom number of mice of the model group in 3 minutes were significantly increased compared with the sham group （ p〈0. 01）;the darkroom number of GM1 group, the HBO group, and HBO＋GM1 group mice were significantly decreased compared with model group （p〈0.05, p〈0.01）, entered the dark room number of HBO ＋GM1 group were returned to normal levels, was statistically signifi-cant compared with the GM1 group and HBO group respectively （p〈0.05）.Water maze test：to reach number of plateau of model group in 10 seconds were significantly reduced compared with the sham group （p〈0.05）;to reach the number of plateau within 10 seconds of HBO＋GM1 group were basic return to normal compared with the model group （p〈0.05）, HBO＋GM1 group was statistically significant compared with the GM1 group and HBO group respectively （p〈0.05）.Morphology of the hippocampus：hippocampal pyramidal cells of HBO＋GM1 group were closely ranged , morphology of most cells was back to normal , cell number and density increased , the role of HBO＋GM1 group significantly increased compared with the GM1 group and HBO group respectively （p〈0.05）.Conclusion：These results suggest that HBO ＋GM1 may play a protective role on the hippocampus tissue morphology change and learn memory dysfunction induced by cerebral ischemia reperfusion in mice , moreover, it is more obvious than the role of GM1 and HBO alone.