穿山龙总皂苷对哮喘小鼠气道重塑及MMP-9、TIMP-1表达的影响

Protective Effect of Con-saponin of Dioscoreae Nipponicae on Asthma-induced Airway Remodeling and Expression of MMP-9 and TIMP-1 in Asthmatic Mice

目的研究穿山龙总皂苷对哮喘小鼠气道重塑和MMP-9、TIMP-1蛋白表达的影响,并探讨其中作用机制。方法将60只清洁级BALB/c小鼠随机分为6组(n=10):A组(空白组),B组(哮喘模型组),C组[阳性对照组(醋酸泼尼松组)],D组(低剂量组),E组(中剂量组),F组(高剂量组)。卵白蛋白(OVA)致敏激发小鼠制作哮喘气道重塑模型,在实验第18-55天,空白组、模型组以生理盐水灌胃,阳性对照组给醋酸泼尼松混悬液10 mg/kg,药物组分别给予穿山龙总皂苷20 mg/kg、40 mg/kg、80 mg/kg治疗。用HE染色法观察各组小鼠肺组织病理形态变化;用蛋白免疫印记法(Western Blot)测定各组MMP-9、TIMP-1蛋白表达。结果穿山龙总皂苷能够改善哮喘小鼠气道壁及上皮结构,减少黏膜下及管壁周围炎性细胞浸润;与空白对照组比较,小鼠哮喘模型组MMP-9表达明显升高(P<0.05),TIMP-1表达明显降低(P<0.05);与模型组比较,各治疗组MMP-9表达明显降低(P<0.05),TIMP-1表达明显升高(P<0.05);MMP-9在各穿山龙总皂苷组表达强于阳性对照组,TIMP-1在各穿山龙总皂苷组表达弱于阳性对照组。结论穿山龙总皂苷能够改善哮喘小鼠气道结构,并可通过抑制MMP-9、增加TIMP-1蛋白的表达从而减轻小鼠哮喘气道重塑状态。

Objective To Study the effect of Con-saponin of Dioscoreae Nipponicae（CDN） on expressions of airway remodeling and to investigate the role of matrix metallo proteinase-9（MMP-9） and its tissue inhibitor（TIMP-1） in asthmatic mice.Methods Sixty SPF BALB/c mice were randomly divided into six group：normal group,model group, the Prednisone therapy group,low-dose CDN group,medium-dose CDN group,high-dose CDN group. The six groups except normal group were established by ovalbumin（OVA） sensitization.During the 18 ~55 days in the experiment,the normal and model groups were treated with normal saline,the Prednisone therapy group were treated with 10mg/kg of prednisone. At the same time,CDN groups were respectively treated with 20mg/kg、40mg/kg、80mg/kg of CDN. The pathological change of lung issues structure was studied under light microscopy and the expression of MMP-9 and TIMP-1 in lung tissues was detected by Western blotting. Results The pathological changes in lung tissue of asthmatic mice showed that CDN could improve the structure of the wall of the airway wall and epithelial,and reduce the infiltration of inflammatory cells. Compared with the normal group,The level of MMP-9 was significantly increased and TIMP-1 was significantly decreased in the model group（P〈0.05）. Compared with the model group,the treatment groups significantly decreased the expression of MMP-9 （P〈0.05） and significantly increased the expression of TIMP-1（P〈0.05）. The level of MMP-9 in all CDN groups was higher than the prednisone therapy group,and the level of TIMP-1 in all CDN groups lower in the prednisone therapy group. Conclusion CDN can improve the airway structure of asthmatic mice,and through inhibiting the expression of MMP-9 and increasing the expression of TIMP-1 to reduce the mice airway remodeling in asthma status.