摘要
目的建立滇紫草、密花滇紫草和昆明滇紫草中乙酰紫草素的含量测定方法。方法采用HPLC法测定滇紫草、密花滇紫草和昆明滇紫草中乙酰紫草素的含量。结果乙酰紫草素在0.0952~1.9030μg范围内呈良好的线性关系。精密度、稳定性、重复性和加样回收率均达到要求。结论该方法简便、准确、重现性好,可用于滇紫草及其两个近缘种中乙酰紫草素含量的测定。
Objective To establish the determination method of acetylshikonin in three species,Onosma paniculatum Bur. et Fr.,O. cingulatum W. W. smith et J. E. Jeffrey and O. confertum W. W. Smith.. Methods Using HPLC as quantitative analysis means and acetylshikonin as the detection of targets,the quality standards of above three species were studied. Results The acetylshikonin had good linear relationship in the range of 0.0952 ~1.9030μg. Precision,stability,repeatability and recovery are achieved. Conclusion The method is simple,accurate,reproducible and suitable for determining the acetylshikonin in Onosma paniculatum and its two closely related species.
出处
《云南中医学院学报》
2014年第6期17-19,22,共4页
Journal of Yunnan University of Traditional Chinese Medicine
关键词
HPLC
滇紫草
乙酰紫草素
HPLC
Onosma paniculatum Bur.et Fr.
acetylshikonin
