红树植物桐花树EF1A基因的克隆与表达分析

Molecular characterization and expression analysis of elongation factor 1 A from mangrove Aegiceras corniculatum

通过RACE方法克隆到桐花树(Aegiceras corniculatum)的一个延伸因子基因,命名为Ac EF1A(Gen Bank登录号:KC416649)。该基因的c DNA全长1 778 bp,CDS为1 350 bp,编码449个氨基酸。多序列比对结果表明该氨基酸序列与琴叶拟南芥(Arabidopsis lyrata)EF1A的氨基酸序列高度相似(97.7%)。基因表达分析结果显示Ac EF1A在茎尖中的表达量最高,叶片中的表达量次之,根中的表达量最低。低温、高盐、干旱和重金属Cd胁迫下,桐花树叶片中Ac EF1A基因的表达水平有不同程度的上调。这些结果表明Ac EF1A基因可能参与了植物的生长发育过程及逆境响应过程。

An elongation factor 1-alpha（EF1A） gene was isolated from mangrove Aegiceras corniculatum and designated as Ac EF1A（Gen Bank accession number： KC416649）. The Full-length c DNA of Ac EF1 A is 1778 bp with a 1350 bp open reading frame（ORF） which encodes 449 amino acids. Multiple sequence alignment revealed that the deduced amino acid sequence of Ac EF1 A shares high similarity with the EF1A（XP_002864684） from Arabidopsis lyrata（97.7%）. Quantitative real-time PCR was employed to determine the expression of Ac EF1 A in different tissues and in response to cold, salt, drought and heavy metal（cadmium, Cd）. Results showed that transcripts of Ac EF1 A were detected in all tissues（leaves, stems and roots） of plantlets, with the highest expression in stems and the lowest in roots. The expression of Ac EF1 A was differentially induced by cold, salt, drought and Cd. These results suggest that the Ac EF1 A may play an essential role in plant development and may be involved in the response to abiotic stresses.