酶碱法提取梨渣水不溶性膳食纤维的研究

Research on extraction of water insoluble dietary fiber from pear residue by alkali enzymic hydrolysis

以梨渣为原料,用酶与碱结合提取的方法,探讨了酶用量、料液比、氢氧化钠溶液浓度、温度和时间对酶碱法提取梨渣水不溶性膳食纤维得率的影响,并对其脱色工艺进行了研究。结果表明,用淀粉酶4 U/g在p H6.0下处理后,在料液比1 g∶15 m L、氢氧化钠溶液浓度1.0 mol/L,温度50℃,时间1 h的条件下提取,梨渣水不溶性膳食纤维的得率最高,达到12.9%。最优的脱色条件是H2O2溶液体积浓度8%,温度60℃,时间3 h。产品的膨胀力、持水力分别达到6.167 g/m L、7.1 g/g。

Water insoluble dietary fiber extracted and decoloration from pear residue by using enzyme method and al-kali extraction method was studied. Effects of enzyme dosage, the ratio of material to solution, alkali concentration, temperature and time on extraction yield of water insoluble dietary fiber were investigated trough single-factor experi-ment method. Subsequently,a central composition experimental design was performed to attain the optimum decoloring project of water insoluble dietary fiber. The optimized conditions were as follows：amylase （4 U/g） at the conditions of pH6.0, and then with the ratio of material to solution 1 g∶15 mL,NaOH concentration 1.0 mol/L,temperature 50℃and time 1 h,the yield of water insoluble dietary fiber was found to be 12.9%. The optimum decoloring conditions were：the concentration of H2O2 8%,the reaction temperature 60℃,the reaction time 3 h. Under the optimal conditions, the expansibility,water-holding capability were 7.1 mL/g,6.167 g/g,respectively.