一种快速制备毕赤酵母表达系统PCR鉴定模板DNA的方法

A Method for Rapid Preparing DNA Template to Identify Pichia pastoris Expression System by PCR Amplification

文章研究了简便高效获取毕赤酵母表达系统PCR鉴定模板DNA的提取方法,采用酶消化和物理冻熔结合的方法,提取的酵母基因组DNA直接PCR扩增检测质量。结果表明,该方法提取酵母基因组DNA具有质量高、成本低、耗时短、操作简单等优点。

In order to obtain a simple and effective method for rapid preparing DNA template to identify Pichia pastoris expression system by PCR amplification, The method of enzyme digestion was combined application with Physics of Freezing ＆ Thawing to extract Pichia pastoris genome DNA, which was detected by PCR as template. The results showed that this method presented advantages of high quality extracts, lower cost, less time consuming and ease of operation.