人参总皂苷的发酵及其产物的抗癌活性研究

Fermentation of ginseng total saponins and anticancer activity of fermentation products

从土壤中分离得到14种β-葡萄糖苷酶高产菌株,其中菌株YS2能够有效地将人参茎叶总皂苷和人参提取物中的人参皂苷转化为稀有人参皂苷Rg3;菌株YS2和YS7的人参发酵物对结肠癌colon26-M3.1细胞具有较强的抑制作用,其IC50值分别为180μg/mL和170μg/mL.根据ITS序列构建的系统发育树,确定菌株YS2为链格孢属(Alternaria).

A total of 14 isolates of high-yieldβ-glycosidase microorganisms were isolated from soil.Among these isolates,strain YS2 shows a strong ability to convert total saponins of ginseng stems and leaves and ginseng extracts to minor ginsenoside Rg3.Fermented ginseng products by strain YS2 and YS7 could strongly inhibit the growth of colon 26-M3.1 cells and the IC50 values are 180μg/mL and 170μg/mL,respectively. Phylogenetic tree analysis based on ITS sequences show that the strain YS2 belongs to the genus Alternaria.