环境水中嗜肺军团菌分离培养与巢式PCR检测研究

Detection of Leginellapneumophiliain environmental water source by conventional culture and nested PCR

目的应用分离培养法与巢式聚合酶链反应法(PCR)检测深圳环境水中军团菌最新污染状况。方法于2012年9-11月采集商场、宾馆和综合性医院三类场所的中央空调冷却水、淋浴水、自来水、景观水,分别用传统分离培养法与巢式聚合酶链反应法(PCR)检测嗜肺军团菌。结果本次共检测环境水样171份,其中传统分离培养法检出64份嗜肺军团菌,巢式聚合酶链反应法(PCR)检出130份嗜肺军团菌,水样的阳性率分别为37.4%和76%。结论深圳多种水源存在军团菌污染;巢式聚合酶链反应法(PCR)检出率高于传统分离培养法。

Objective To detect the contamination by Legionella pneumophila in environmental water source of Shenzhen by conventional culture and nested PCR. Methods Cooling water, shower warm water, tap water and landscape Water from stores, hotels and hospitals were collected during September and November, 2012. Leginella pneumophilia was tested by conv- entional culture and nested PCR . Results Totally, 171 samples were collected. LegioneUa pneumophila was found in 64 samples by conventional culture and in 130 samples by nested PCR. The positive rates of Legionella pneumophila by the two methods were 37.4 % and 76.0 %, respectively. Conclusions Legionella pneurnophilia exists in environmental water source of Shenzhen. Detection rate by nested PCR is higher than that by conventional culture.