响应面法优化莲藕中结合态阿魏酸的提取工艺

Optimization of Alkaline Hydrolysis of Lotus Root for Bound Ferulic Acid by Response Surface Methodology

为从莲藕中提取出结合态阿魏酸,首先用质量分数75%乙醇除去莲藕中游离态阿魏酸,再用NaOH溶液提取结合态阿魏酸,紫外分光光度法在324 nm波长处测定其含量。通过单因素试验对影响结合态阿魏酸提取的4个因素即NaOH浓度、提取温度、提取时间、液固比进行初步优化分析,再通过Box-Behnken试验设计和响应面优化最终提取条件。结果表明:NaOH溶液浓度1.49 mol/L、提取时间2.66 h、提取温度84.45℃、液固比5.5∶1(mL/g)为最佳提取工艺,在此条件下,莲藕中结合态阿魏酸提取率为0.217 mg/g(以干质量计)。

In this work, bound ferulic acid （FA） was extracted from lotus root with NaOH solution following the removal of free FA with 75% ethanol solution. FA content was spectrometrically determined at 324 nm, where it had characteristic absorption. A preliminary investigation into four extraction parameters including NaOH concentration, temperature, extraction time and solvent-to-solid ratio was carried out by one-factor-at-a-time design. By using Box-Behnken design and response surface analysis, the optimal extraction conditions were established as follows： NaOH concentration, 1.49 mol/L; extraction time, 2.66 h; extraction temperature, 84.45℃, and solvent-to-solid ratio, 5.5：1 （mL/g）. Under the optimized conditions, the yield of found FA from lotus root was 0.217 mg/g （by dry weight）.