响应面法优化酶辅助闪式提取二氢槲皮素工艺

Optimization of Cellulase-Assisted Extraction of Dihydroquercetin by Response Surface Methodology

目的:利用响应面法对纤维素酶辅助闪式提取二氢槲皮素的工艺条件进行优化。方法:以二氢槲皮素的提取率为指标,在单因素试验的基础上,应用Box-Behnken试验设计,对影响提取率的条件(乙醇体积分数、加酶量和液料比)进行优化。结果:最佳提取条件为乙醇体积分数60.93%、加酶量3.48 mg/g、液料比103.64∶1(mL/g)、提取时间120 s。结论:闪式提取操作简便、节能、省时,可应用于二氢槲皮素大规模生产。

Objective： To optimize the celluase-assisted extraction of dihydroquercetin from Larix olgensis Henry var. koreana Nakai wood chips by response surface methodology （RSM）. Methods： Dihydroquercetin was extracted with ethanol in a herbal blitzkrieg extractor from pine wood chips pre-hydrolyzed with celluase. On the basis of the results of single-factor experiments, a Box-Behnken design was performed to optimize three extraction conditions including ethanol concentration, enzyme dosage and liquid-to-solid ratio. Results： The optimal extraction conditions were determined as hydrolysis with an enzyme dosage of 3.48 mg/g and subsequent extraction for 120 s with 60.93% ethanol in water at a liquid-to-solid ratio of 103.64：1 （mL/g）. Conclusion： The proposed extraction procedure can provide a simple, energy-saving and time-saving way for massive production of dihydroquercetin.