顶空固相微萃取-气相色谱-质谱联用法分析仔姜与老姜的挥发性成分

Analysis of Volatile Compounds in Early and Late Harvested Ginger by Headspace-Solid Phase Micro-ExtractionGas Chromatography-Mass Spectrometry

以仔姜和老姜为原料,采用顶空固相微萃取-气相色谱-质谱联用法、以正癸烷为内标,分别对仔姜和老姜的挥发性成分进行分析。研究结果表明:从仔姜和老姜中共鉴定出89种挥发性成分,其中仔姜中鉴定出63种挥发性成分,主要包括37种烃类、15种醇类、6种酯类、3种醛类、2种酮类,其主要挥发性成分为姜烯、橙花醇乙酸酯、β-雪松烯、α-法尼烯、(E)-柠檬醛、β-红没药烯;老姜中鉴定出68种挥发性成分,主要包括45种烃类、14种醇类、3种酯类、3种醛类、3种酮类,其主要挥发性成分为姜烯、α-姜黄烯、β-雪松烯、α-法尼烯、(E)-柠檬醛、β-红没药烯。

The volatile compounds in early and late harvested ginger （Zingiber officinale Roscoe） were analyzed by headspace-solid phase micro-extraction （HS-SPME） combined with GC-MS using decane as an internal standard. A total of 89 volatile compounds were identified in ginger. There were 63 volatiles compounds in early harvested ginger （in August） including 37 hydrocarbons, 15 alcohols, 6 esters, 3 aldehydes and 2 ketones, whereas there were 68 volatiles compounds in late harvested ginger （in September of the same year） including 45 hydrocarbons, 14 alcohols, 3 esters, 3 aldehydes and 3 ketones. The major volatile compounds in early harvested ginger included zingiberene, neryl acetate, beta-cedrene, alpha- farnesene, trans-citral and beta-bisabolene, while those identified in late harvested ginger included zingiberene, alpha- curcumene, beta-cedrene, alpha-farnesene, trans-citral and beta-bisabolene.