小鼠H22肝癌细胞株淋巴结转移模型的构建

Establishment of lymph node metastasis models with hepatocellular carcinoma H22 cells

目的:使用H22肝癌细胞株建立小鼠自发淋巴道转移模型。方法:40只雄性昆明种小鼠随机分为4组(n=10),左后肢爪垫皮下接种H22肝癌细胞建立动物模型,各组小鼠接种细胞浓度分别为:1×106·只-1、5×105·只-1、2.5×105·只-1,对照组注射等体积PBS。接种后第27天解剖取小鼠爪垫原发灶肿瘤组织及腘窝部淋巴结,10%中性福尔马林液固定、HE染色后判断淋巴结转移情况,计算转移率。结果:高浓度组(1×106·只-1)转移率为100%,中浓度组(5×105·只-1)转移率为50%,低浓度组(2.5×105·只-1)转移率为40%。结论:小鼠爪垫皮下接种1×106的H22肝癌细胞株,可以建立100%腘窝部淋巴结转移模型。

Objective： To establish spontaneous lymph node metastasis models with murine hepatocellular carcinoma H22 cells. Methods. H22 cells were injected subcutaneously into the hind-paw of Kunming mice. Each group（n=10） received different number of cells： 1 10^6 per mouse, 5 10^5 per mouse, 2. 5 10^5 per mouse. After 27 days, paw tumor tissue and popliteal fossa lymph nodes were removed, fixed in 10% neutral formalin, and stained with H＆E for histopathological studies. Results： Lymph node with cancer cells was defined as positive metastasis model and the metastasis rate of each group was calculated then. As it showed that the lymph node metastasis rate were 100%（group H： 1 10^6 per mouse）, 50% （group M： 5 10^5 per mouse）, and 40% （group L： 2. 5 10^5 per mouse）, respectively. Conclusion. Our results indicated that mice injected with 1 108 H22 cells subcutaneously can establish a 100% lymph node metastasis model.