STEAP基因在胃癌组织中表达与淋巴结转移的关系

Association of Up-regulation of STEAP Gene Expression with Lymph Node Metastasis in Gastric Cancer

目的探讨前列腺六跨膜上皮抗原（STEAP）基因 mRNA 表达在胃癌发病过程的意义。方法收集28例新鲜胃癌组织,21例胃癌旁组织以及16例胃癌淋巴结转移组织。常规 Trizol 方法提取组织总 RNA,将这些总RNA反转录成cDNA后,利用实时荧光定量PCR检测STEAP 基因表达。统计分析STEAP基因表达变化与胃癌发病及淋巴结转移的关系。结果与癌旁胃组织相比,STEAP 基因在胃癌组织中表达明显增高。与胃癌淋巴结转移组织相比,STEAP基因在胃癌组织中表达则相应降低。结论 STEAP基因在胃癌中表达上调,并促进了胃癌淋巴结转移。

Obj ective]To explore the significance of the expression of STEAP mRNA in the pathogenesis of gastric cancer.[Methods]Totally 28 cases of fresh gastric cancer tissue,21 cases of peri-cancer gastric tissue and 1 6 cases of lymph node metastasis tissue of gastric cancer were collected.Total RNA was extracted by routine Tr-izol way.After total RNAs were transcribed into cDNA,the expression of STEAP gene was detected by real-time PCR.The correlation of STEAP expression with the pathogenesis and lymph node metastasis of gastric cancer was analyzed by statistical method.[Results]Compared with normal gastric tissues,the expression of STEAP in gas-tric cancer tissues was obviously increased.Compared with lymph node metastasis tissue,the expression of STEAP in gastric cancer tissue was decreased.[Conclusion]The expression of STEAP in gastric cancer is up reg-ulated and can promote lymph node metastasis of gastric cancer.