吸烟对男性精子形态精子DNA完整率和精子顶体完整率的研究

Effect of smoking on the male sperm morphology,sperm DNA integrity rate and sperm acrosome integrity rate

目的：研究吸烟对男性精子形态、精子DNA完整率[用精子DNA断裂指数（ DFI）表示]及顶体完整率的影响。方法回顾性分析来该中心就诊的1500例男性患者（1300例吸烟者及200例未吸烟者），按每天吸烟量分为A1组500例，＞20支／d；A2组600例，10-20支／d；A3组200例，1-9支／d。 B组200例，0支／d，作为对照组。结果精子正常形态随着每天吸烟量的增加而下降，各组间两两比较差异有统计学意义（ P＜0．05）。精子DNA完整率随着吸烟量的上升而降低（ DFI升高），各组间两两比较差异有统计学意义（ P＜0．05）。精子顶体完整率随吸烟量上升而下降，各组间两两比较差异有统计学意义（ P＜0．05）。结论随着每天吸烟量增加精子正常形态、精子DNA完整率及精子顶体完整率均降低，不利于男性生殖健康。

Objective To study the effect of smoking on male sperm morphology ,sperm DNA integrity rate [ DNA fragmentation index （ DFI） was used for the evaluating ] and acrosome integrity rate .Methods A retrospective analysis was performed on 1 500 male patients come to our center for treatment （ 1 300 smokers and 200 non-smok-ers）.According to the daily smoking quantity the patients were divided into A 1 group（500 cases）：〉20/day, A2 group（600 cases）：10-20/day, A3 group（200 cases）：1-9/day;and group B（200 cases）：0/day（as the control group） .Results The normal morphology rate of sperm decreased with the increase of daily smoking quantity , the pair comparison showed a significant differences between groups （ P〈0.05 ）; the rate of sperm DNA integrity de-creased with the rise of smoking （ DFI will be increase ） , the pair comparison showed a significant differences between groups（P〈0.05）;the sperm acrosome integrity rate decreased with the increase of smoking quantity , the pair com-parison showed a significant differences between groups （ P〈0.05 ） .Conclusion With the increase of daily smoking quantity the rate of normal sperm morphology , sperm DNA integrity rate and sperm acrosome integrity rate are re-duced,and is not good for male reproductive health .