摘要
根据已知植物的苹果酸脱氢酶基因序列设计简并引物,采用RT-PCR技术从花生叶片中克隆得到苹果酸脱氢酶基因,命名为Ah MDH,Genbank登录号为KF499119,该基因编码区长度为1071bp,编码356个氨基酸。序列比对结果表明,Ah MDH编码蛋白与大豆、蒺藜苜蓿和豌豆等具有较高的相似性。
In this paper, degenerate primers were designed according to reported malate dehyrogenase gene sequences. The coding sequence of malate dehyrogenase gene（Ah MDH） numbered KF499119 was amplified by RT-PCR from c DNA of Arachis hypogaea L. The sequence of Ah MDH is 1071 bp in length encoding 356 amino acid. Multiple sequence alignment analysis showed that A. hypogaea, Glycine max, Medicago truncatula and Pisum sativum share high homology.
出处
《台州学院学报》
2014年第6期38-44,共7页
Journal of Taizhou University
基金
浙江省教育厅科研项目(Y201223322)
2014年浙江省大学生科技创新活动计划(新苗人才计划)(2014R428013)
关键词
花生
苹果酸脱氢酶
克隆
序列分析
Arachis hypogaea
malate dehyrogenase
gene cloning
sequence analysis
