摘要
目的按照修订的硫代硫酸盐-柠檬酸盐-胆盐-蔗糖(TCBS)琼脂培养基行业标准中的要求进行试验,验证该行业标准的适用性。方法取不同厂家生产的TCBS琼脂培养基,根据TCBS琼脂培养基行业标准的要求,进行pH值、水分的测定和微生物生长试验。测定了TCBS琼脂培养基的pH值和水分,并将质控菌株的培养物接种到受试的培养基平皿中进行微生物生长试验。结果 TCBS琼脂培养基的pH值和水分均符合行业标准的规定,且各质控菌株生长良好。结论作为推荐性的国家行业标准,TCBS琼脂培养基行业标准可以用于我国该培养基的质量评价和监管工作的需要。
Objective To verify the application of professional standard for Thiosulfate-Citrate-Bile Salts-Sucrose（TCBS）agar medium.Methods TCBS agar medium produced by different factories are obtained for pH value and microbiological grow test according to the formulated medium standard.pH value and solution of medium are determined and bacterial cultures of the control strains are inoculated in the medium to detected bacterial growth.Results pH value and solution of medium were within the specified range.The control strains grew well.Conclusion As the recommended industrial standard,TCBS agar medium standard may be applied to evaluate and supervise the TCBS agar medium quality in our country.
出处
《中国药事》
CAS
2014年第12期1367-1370,共4页
Chinese Pharmaceutical Affairs
关键词
培养基
质控菌株
理化试验
微生物生长试验
medium
control strains
physical and chemical experiments
microbiological grow test
