B细胞非霍奇金淋巴瘤患者外周血CD4＋CD25high CD127low调节性T细胞水平及其临床意义

Detection of peripheral blood CD4＋ CD25high CD127low regulatory T cells in patients with B-cell non-Hodgkin lymphoma and its clinical significance

目的 探讨外周血CD4＋ CD25high CD127low调节性T细胞（Treg细胞）在B细胞非霍奇金淋巴瘤（B-NHL）患者外周血中表达水平及其临床意义.方法 采用流式细胞术检测100例初诊B-NHL患者及50名健康对照者外周血CD4＋ CD25high CD127low Treg细胞表达水平,进行统计学分析.结果 健康对照者外周血CD4＋ CD25high CD127low Treg细胞中位表达水平为5.00％,初诊B-NHL患者为7.20％,两者之间差异具有统计学意义（P＜ 0.001）.男性患者外周血CD4＋ CD25high CD 127low Treg细胞水平高于女性患者（P＜0.01）,乳酸脱氢酶（LDH）增高患者外周血CD4＋ CD25high CD127low Treg细胞水平高于LDH正常患者（P＜0.01）,Ⅲ～Ⅳ期患者外周血CD4＋C D25high CD 127low Treg细胞水平较Ⅰ～Ⅱ期患者增高（P＜0.01）,有B症状患者外周血CD4＋ CD25high CD127low Treg细胞水平高于无B症状患者（P＜0.01）.而不同年龄、国际预后指数评分、有无大包块患者之间外周血CD4＋ CD25high CD 127low Treg细胞水平差异均无统计学意义（均P＞0.05）.结论 B-NHL患者体内存在免疫抑制,在男性、LDH增高、有B症状及晚期患者中CD4＋ CD25high CD127low Treg细胞水平明显增高.检测其水平对于判断B-NHL的预后有一定价值.

Objective To detect peripheral blood level of CD4＋ CD25high CD127low regulatory T （Treg） cells in patients with B cell non-Hodgkin lymphoma （B-NHL） and to explore its clinical significance.Methods The levels of peripheral blood CD4＋ CD25high CD127low Treg cells of 100 newly diagnosed patients with B-NHL and 50 healthy adults were detected and analyzed by flow cytometry.Results The levels of peripheral blood CD4＋ CD25high CD12low Treg cells were 5.00 % and 7.20 % in healthy adults and newly diagnosed B-NHL patients,respectively.The difference was statistically significant （P 〈 0.001）.The level of peripheral blood CD4＋ CD25high CD127low Treg cells was significantly higher in the male patients than that in the female （P 〈 0.01）.The level of peripheral blood CD4＋ CD25high CD127low Treg cells was significantly higher in elevated LDH patients than that in normal LDH patients （P 〈 0.01）.The level of peripheral blood CD4＋ CD25high CD127low Treg cells in stage Ⅲ-Ⅳ patients was significantly higher than that in stage Ⅰ-Ⅱ patients （P 〈 0.01）.The level of peripheral blood CD4＋ CD25high CD127low Treg cells in B symptom patients was significantly higher than that in no B symptom patients （P 〈 0.01）.There was no significant relationship between the level of peripheral blood Treg cells and age,IPI score,bulky disease or pathologic subtype （all P 〉 0.05）.Conclusions B-NHL patients were in an immunosuppresive statue.Male,elevated LDH level,B symptom and Ⅲ-Ⅳ stage were correlated with higher level of peripheral blood CD4＋ CD25high CD127low Treg cells.Level of peripheral blood Treg cells may be a useful prognostic factor for B-NHL.