响应面法优化锁阳熊果酸提取工艺

Optimization of Extraction Technology of Ursolic Acid from Cynomorium Songaricum Rupr. with Response Surface Method

对锁阳熊果酸的乙醇回流提取工艺进行了研究,并采用响应面法优化了提取工艺条件。结果表明:在乙醇体积分数为80%、提取温度78℃、提取时间1.6 h、液料比14∶1(m L∶g)、提取2次的条件下,锁阳熊果酸的提取量为6.43 mg/g,提取率为97.72%。

The ethanol refluxing extraction technology of ursolic acid from Cynomorium songaricum Rupr. was studied. The response surface method was used to optimize the conditions. The result indicate that under conditions of ethanol volume fraction 80%, extraction temperature 78℃, extraction time 1.6 h, liquidsolid ratio 14 ∶ 1 （ mL ∶ g） , extraction times 2, the extraction amount of ursolic acid was 643 mg/g and the extraction rate was 97.72%.