利用SSR标记进行杂交油菜品种鉴定

Identification of hybrid rapeseed using SSR markers

利用分布于油菜连锁群上的33对SSR核心引物,对12个育种品系和市场上的杂交油菜品种进行鉴定,探讨取样方案、检测方法和位点判读,将样品间SSR位点类型分为相同位点、疑似相同位点和差异位点3类。分析位点类型发现,12个油菜品种/系间的差异位点数为4~22,其中同父异母杂交种（3个）间差异位点数为4~16,同母异父杂交种（4个）间差异位点数为7~18,市场抽样的品种间差异位点数为12~20。同一品种不同样本间的差异位点数为0~2,据此,将区分品种间与品种内的差异位点阈值设定为2,即在利用33对引物对油菜杂交种与对照样品进行SSR分析时,当两样品间差异位点数〉2时,判定为不同品种;当差异位点数≤2时,判定为近似或极近似品种。盲样鉴定实验及其与田间鉴定方法的对比实验,均证明了本研究确定的SSR鉴定方法可行。

For new hybrid rapeseed （Brassica napus L. ） identification, previous 33 pairs of SSR markers were used to identify 12 breeding lines and commercial euhivars. Results showed that 3 patterns of SSR markers types with 4 -22 distinct markers were obtained from the samples. They were identical type, suspected identical type and distinct type. Among the 12 materials, 3 half- sib hybrids with the same paternal parent exhibited 4 -16 distinct markers, and 4 half - sib hybrids with the same maternal parent exhibited 7 - 18 distinct markers. Further- more, among the commercial hybrids from market, 12 -20 distinct markers were found. 0 -2 distinct markers occurred between 2 samples with the same hybrid. Therefore, for identifying genuineness of rapeseed hybrids by using 33 SSR markers, the sample could be recognized as the same hybrid or very similar hybrid if the distinct markers were less than 2. That would be the threshold for identifying as different hybrids. The feasibility of this method was further proved by blind identification.