曝气对栅藻(Desmodesmus sp.CHX1)细胞生长和油脂生产的影响

Effects of aeration on growth and lipid productivity of Desmodesmus sp.CHX1

在自制生物反应器内,考察了曝气对培养于BG-11培养液中的栅藻(Desmodesmus sp.CHX1)细胞生长、叶绿素积累和油脂生产的影响。结果表明:曝气能显著促进藻细胞生长、增加细胞内叶绿素(a+b)含量和油脂产量,当曝气量为160L/h时达到最大。培养7d后,栅藻细胞的生物质浓度、叶绿素(a+b)含量和油脂产率分别达到7.26g/L、60.5mg/L和128.7mg/(L·d),显著高于不曝气对照的0.47g/L、2.81mg/L、39.9 mg/(L·d)。由此说明在微藻培养过程中,曝气利于栅藻细胞生长、叶绿素积累和油脂生产,这为强化栅藻细胞生长和油脂生产提供了一种途径。

To promote growth and lipid production of microalgae for biodiesel, aeration effect was studied on growth of a microalga Desmodesmus sp. CI-IX1 cultured in BG - 11 medium. Results showed that aeration could dramatically increase the algal biomass, chlorophyll （a ＋ b） content and lipid productivity. After 7d cultivation with the aeration 160L/h, the maximum biomass of CHX1 cell, chlorophyll （a ＋ b） content and lipid productivity reached 7.26g/L, 60.5mg/L and 128.7mg/（L d） respectively, which were significantly higher than those of control. Results suggested that aeration was favorable for the growth of microalgae and accumulation of valuable additional materials during the cultivation of Desmodesmus sp. CHX1, and was also for enhancement of cell growth and lipid production.