花生根系保护酶对不同生育期淹涝的响应

Response of root protective enzymes to waterlogging at different growth stages of peanut

采用人工模拟根际淹水方法,以典型耐淹涝花生品种豫花15、淹涝敏感品种花269为材料,研究根系保护酶(SOD、POD、CAT)、膜脂过氧化代谢产物丙二醛(MDA)对不同生育期淹水胁迫的响应。结果表明,两个品种在不同生育期对淹涝的响应存在较大差异。苗期淹涝后,豫花15的SOD、POD、CAT活性均下降,MDA含量升高;花269的SOD活性先升后降,CAT活性先降后升,POD活性、MDA含量升高。花针期淹涝后,豫花15的SOD、POD、CAT活性均先升后降,MDA含量降低;花269的SOD和CAT活性、MDA含量均先降后升,POD活性升高。结论:淹涝对花生根系内在生理有负面影响,尤以苗期影响大,花针期影响较小;而品种间根系保护酶(SOD、POD、CAT)的活性变化较为复杂;苗期豫花15根系耐淹涝性弱于花269,而在花针期强于花269。

In order to research the response of root protective enzyme （SOD, POD, CAT） and MDA on the different growth stages of 2 different typical yield waterlogging tolerance peanut varieties, Yuhua 15 （waterlogging tolerance） and Hua 269 （sensitive）, simulation experiment was used under rhizosphere waterlogged. Results showed that, there was a big difference in the response of waterlogging on different varieties and growth stages of peanut. SOD, POD and CAT activity declined, but MDA content increased in Yuhua 15; SOD activity first increased and then decreased, but CAT activity first decreased and then increased, while POD activity and MDA content increased in Hua 269 after peanut waterlogged at the seedling stage. SOD, POD and CAT activity first increased and then decreased, while MDA content reduced in Yuhua 15; SOD, CAT activity and MDA content first decreased and then increased, yet POD activity increased in Hua 269 after peanut waterlogged at the flowering stage. Conclusion： Waterlogging had a negative impact on root internal physiology, and it had greater impact in seedling stage than that in the flowering stage. The waterlogging tolerance of Yuhua 15 was weaker than Hua 269 in seedling stage but stronger in flowering stage, while the activities of protective enzymes （ SOD, POD, CAT） appeared more complicated and less predictable.