期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

BK通道在血管平滑肌细胞迁移中的作用

The role of BK channel in the migration of vascular smooth muscle cell
下载PDF
导出
摘要 观察BK通道在血管平滑肌细胞(vascular smooth muscle cell,VSMC)上的功能及数量发生变化时,对VSMC迁移速度的影响。用脂质体转染法使BK通道在VSMC上过表达,或用BK通道特异性阻断剂IBTX(iberiotoxin)阻断VSMC上BK通道的功能,在这两种情形下,观察VSMC迁移速度的变化。研究结果表明,当过表达BK通道于VSMC,与正常对照相比,其迁移速度明显减慢;反之,当BK通道功能被特异性阻断剂IBTX阻断后,VSMC的迁移速度明显加快。最后认为BK通道的表达水平与VSMC的迁移速度呈负相关,过表达BK通道抑制VSMC的迁移。 To investigate the effect of BK channel on migration speed of the vascular smooth muscle cells （VSMC） , when the function and amount of BK channels on VSMC altered. Over-expressed BK channels on VSMC by liposome transfeetion, or blocked the function of BK channels on VSMC by specific blocker iberiotoxin （IBTX） , so as to observe the change of VSMC migration speed in the two conditions. Over-expressed BK channel on VSMC obviously decreased migration speed of VSMC compared with normal control, on the contrary, the migration speed increased when the BK channels were blocked by IBTX. The expression level of BK channel is negatively related with the migration speed of VSMC. Over-expressed BK channel on VSMC inhibited the migration speed.
作者 李晨旭 马国英 杨靖辉 刘颖
机构地区 山西大同大学医学院生理教研室
出处 《西北大学学报（自然科学版）》 CAS CSCD 北大核心 2014年第6期930-933,共4页 Journal of Northwest University（Natural Science Edition）
基金 山西省自然科学基金资助项目(2012011042-3)
关键词 BK通道 血管平滑肌细胞 迁移 BK channel vascular smooth muscle cell （VSMC） migration
分类号 R363.1 [医药卫生—病理学]
  • 相关文献

参考文献13

  • 1BILATO C,PAULY R R,MELILLO,et al.Intracellular signaling pathways required for rat vascular smooth muscle cell migration-interaction between basic frbroblast growth factor and platelet derived growth factor[J].Journal of Clinical Investigation,1995,96:1905-1915.
  • 2HAUCK C R,HSIA D A,SCHLAEPFER D D.Focal adhesion kinase facilitates platelet-derived growth factor-BB-stimulated ERK2 activation required for chemotaxis migration of vascular smooth muscle cells[J].Journal of Biological Chemistry,2000,275:41092-41099.
  • 3KUSCH A,TKACHUK S,HALLER H.Urokinase stimulates human vascular smooth muscle cell migration via a phosphatidylinositol 3-kinase-Tyk2 interaction[J].Journal of Biological Chemistry,2000,275:39466-39473.
  • 4ZHAO H C,AGULA H,ZHANG W,et al.Membrane stretch and cytoplasmic Ca2+independently modulate stretch-activated BK channel activity[J].Journal of Biomechanics,2010,43:3015-3019.
  • 5ZHAO H C,WANG F.Exercise training changes the gating properties of large-conductance Ca2+-activated K+channels in rat thoracic aorta smooth muscle cells[J].Journal of Biomechanics,2010,43:263-267.
  • 6OHYA S,YAMAMURA H,MURAKI K,et al.Comparative study of the molecular and functional expression of L-type Ca2+channels and large-conductance,Ca2+-activated K+channels in rabbit aorta and vas deferens smooth muscle[J].Pflugers Archiv-european Journal of Physioligy,2001,441:611-620.
  • 7GRIBKOFF V K,STARRETT J E,DWORETZKY S I.Targeting acute ischemic stroke with a calcium-sensitive opener of maxi-K potassium channels[J].Nature Medicine,2001,7:471-477.
  • 8CHANG TJ,WU L Y,WANG R.Altered expression of BK channel beta(1)subunit in vascular tissues from spontaneously hypertensive rats[J].American Journal of Hypertension,2006,19:678-685.
  • 9Hucheng Zhao Masahiro Sokabe.Tuning the mechanosensitivity of a BK channel by changing the linker length[J].Cell Research,2008,18(8):871-878. 被引量：6
  • 10BINGHAM J P,BIAN S,TAN Z Y,et al.Synthesis of a biotin derivative of iberiotoxin:Binding interactions with streptavidin and the BKCa2+-activated K+channel expressed in a human cell line[J].Bioconjugate Chemistry,2006,17:689-699.

二级参考文献33

  • 1Gustin MC, Zhou XL, Martinac B, Kung C. A mechanosensitive ion channel in the yeast plasma membrane. Science 1988; 242:762-765.
  • 2Qi z, Chi SP, Su XX, Naruse KJ, Sokabe M. Activation a mechanosensitive BK channel by membrane stress created with amphipaths. Mol Membr Bio12005; 22:519-527.
  • 3KlodaA, Lua L, Hall R, Adams D J, Martinac B. Liposome reconstitution and modulation of recombinant N-methyl-D-aspartate receptor channels by membrane stretch. Proc NatlAcadSci USA 2007; 104:1540-1545.
  • 4Hamill OP, Martinac B. Molecular basis ofmechanotransduction in living cell. Physiol Rev 2001 ; 81:685-740.
  • 5Bahn YS, Xue CY, Idnurm A, Rutherford JC, Heitman J, Cardenas MF. Sensing the environment: lessons from fungi. Nat Rev Microbio12007; 5: 57-69.
  • 6Moser T, Brandt A, Lysakowski A. Hair cell ribbon synapses. Cell Tissue Res 2006; 326:347-359.
  • 7Christensen O. Mediation of cell volume regulation by Ca^2+ influx through stretch-activated channels. Nature 1987; 330:66-68.
  • 8Welsh MJ, Price MP, Xie J. Biochemical basis of touch perception: mechanosensory function of degenerin/epithelial Na^+ channels. JBiol Chem 2002; 277:2369-2372.
  • 9Gasull X, Ferrer E, Llobet A, et al. Cell membrane stretch modulates the high-conductance Ca(2+)-activated K^+ channel in bovine trabecular meshwork cells. Invest Ophthalmol Vis Sci 2003; 44:706-714.
  • 10Lee J, Ishihara A, Oxford G, Johson B, Jacobson K. Regulation of cell movement is mediated by stretch-activated calcium channels. Nature 1999; 400:382-386.

共引文献5

西北大学学报（自然科学版）
2014年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部